Induction of oral tolerance in TGF-β1 null mice

K. Siobhan Barone, Dana D. Tolarova, Ilona Ormsby, Thomas C Doetschman, J. Gabriel Michael

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Abstract

Previous studies have suggested that oral tolerance induction by low doses of Ag is mediated by inhibitory cytokines, particularly TGF-β1. To examine the roles of TGF-β1 and other inhibitory cytokines in the induction of oral tolerance, TGF-β1 null mice and controls were gavaged with 10 to 20 mg (high dose) or 1 mg (low dose) of OVA for 3 days. After immunization with OVA, the in vitro proliferative response of OVA-specific popliteal lymph node cells was assessed. Lymphocytes from all TGF-β1 null mice fed high doses of OVA exhibited highly significant suppression compared with controls. A weaker, but still significant, suppression was observed in lymphocytes from the majority of TGF-β1 null mice fed low doses of OVA. In addition, supernatants from these lymphocytes exhibited lower levels of IL-4, IL-10, and IFN-γ than those from water-fed control animals. These results indicate that while TGF-β1 may play a role in suppression, inhibitory cytokines are not the exclusive mechanism by which low dose oral tolerance is induced.

Original languageEnglish (US)
Pages (from-to)154-160
Number of pages7
JournalJournal of Immunology
Volume161
Issue number1
StatePublished - 1998
Externally publishedYes

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Lymphocytes
Cytokines
Interleukin-4
Interleukin-10
Immunization
Lymph Nodes
Water
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

Siobhan Barone, K., Tolarova, D. D., Ormsby, I., Doetschman, T. C., & Gabriel Michael, J. (1998). Induction of oral tolerance in TGF-β1 null mice. Journal of Immunology, 161(1), 154-160.

Induction of oral tolerance in TGF-β1 null mice. / Siobhan Barone, K.; Tolarova, Dana D.; Ormsby, Ilona; Doetschman, Thomas C; Gabriel Michael, J.

In: Journal of Immunology, Vol. 161, No. 1, 1998, p. 154-160.

Research output: Contribution to journalArticle

Siobhan Barone, K, Tolarova, DD, Ormsby, I, Doetschman, TC & Gabriel Michael, J 1998, 'Induction of oral tolerance in TGF-β1 null mice', Journal of Immunology, vol. 161, no. 1, pp. 154-160.
Siobhan Barone K, Tolarova DD, Ormsby I, Doetschman TC, Gabriel Michael J. Induction of oral tolerance in TGF-β1 null mice. Journal of Immunology. 1998;161(1):154-160.
Siobhan Barone, K. ; Tolarova, Dana D. ; Ormsby, Ilona ; Doetschman, Thomas C ; Gabriel Michael, J. / Induction of oral tolerance in TGF-β1 null mice. In: Journal of Immunology. 1998 ; Vol. 161, No. 1. pp. 154-160.
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