Induction of virulence factors, apoptosis, and cytokines in precision-cut hamster liver slices infected with Entamoeba histolytica.

Pilar Carranza-Rosales, María Guadalupe Santiago-Mauricio, Nancy Elena Guzmán-Delgado, Javier Vargas-Villarreal, Gerardo Lozano-Garza, Ezequiel Viveros-Valdez, Rocío Ortiz-López, Javier Morán-Martínez, A Jay Gandolfi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Precision-cut liver slices (PCLS) are mainly used to evaluate hepatotoxicity and metabolism of chemicals, as well as to study mechanisms of liver damage and repair. However, recently they have been used as a system to study amoebic infections. The aim of this study was to validate this model as an alternative for experimental amoebic liver absess (ALA) in animals. To do this, the PCLS was analyzed for the expression of amoebapore and cysteine proteinases 1 and 5, three of the most studied virulence factors of Entamoeba histolytica, as well as the induction of apoptosis and cytokines production in response to the ex vivo infection. PCHLS were prepared with the Brendel-Vitron tissue slicer and then, infected with 200,000 trophozoites of E. histolytica. Samples were taken at 0, 6, 12, 18, and 24 h and compared to control non-infected slices. Morphological studies were performed in order to verify the infection; while apoptosis was studied by TUNEL and PAS techniques. The expression of cysteine proteinases (1 and 5), and amoebapore, was analyzed by real-time PCR. By using ELISA assays, the production of cytokines was also studied. PCHLS were found to be a reproducible infection system, and E. histolytica caused the expression of cysteine proteinases and amoebapore in infected slices. At the same time, trophozoites induce release of cytokines and apoptotic death of the hepatocytes close to them. PCHLS represent a new and suitable alternative model to study the pathogenesis of hepatic amoebiasis.

Original languageEnglish (US)
Pages (from-to)424-433
Number of pages10
JournalExperimental Parasitology
Volume132
Issue number4
StatePublished - Dec 2012
Externally publishedYes

Fingerprint

Entamoeba histolytica
Virulence Factors
Cricetinae
Cysteine Proteases
Apoptosis
Cytokines
Trophozoites
Liver
Infection
Amoebic Liver Abscess
In Situ Nick-End Labeling
Real-Time Polymerase Chain Reaction
Hepatocytes
Enzyme-Linked Immunosorbent Assay
protozoan amoebapore proteins

ASJC Scopus subject areas

  • Parasitology
  • Immunology

Cite this

Carranza-Rosales, P., Santiago-Mauricio, M. G., Guzmán-Delgado, N. E., Vargas-Villarreal, J., Lozano-Garza, G., Viveros-Valdez, E., ... Gandolfi, A. J. (2012). Induction of virulence factors, apoptosis, and cytokines in precision-cut hamster liver slices infected with Entamoeba histolytica. Experimental Parasitology, 132(4), 424-433.

Induction of virulence factors, apoptosis, and cytokines in precision-cut hamster liver slices infected with Entamoeba histolytica. / Carranza-Rosales, Pilar; Santiago-Mauricio, María Guadalupe; Guzmán-Delgado, Nancy Elena; Vargas-Villarreal, Javier; Lozano-Garza, Gerardo; Viveros-Valdez, Ezequiel; Ortiz-López, Rocío; Morán-Martínez, Javier; Gandolfi, A Jay.

In: Experimental Parasitology, Vol. 132, No. 4, 12.2012, p. 424-433.

Research output: Contribution to journalArticle

Carranza-Rosales, P, Santiago-Mauricio, MG, Guzmán-Delgado, NE, Vargas-Villarreal, J, Lozano-Garza, G, Viveros-Valdez, E, Ortiz-López, R, Morán-Martínez, J & Gandolfi, AJ 2012, 'Induction of virulence factors, apoptosis, and cytokines in precision-cut hamster liver slices infected with Entamoeba histolytica.', Experimental Parasitology, vol. 132, no. 4, pp. 424-433.
Carranza-Rosales P, Santiago-Mauricio MG, Guzmán-Delgado NE, Vargas-Villarreal J, Lozano-Garza G, Viveros-Valdez E et al. Induction of virulence factors, apoptosis, and cytokines in precision-cut hamster liver slices infected with Entamoeba histolytica. Experimental Parasitology. 2012 Dec;132(4):424-433.
Carranza-Rosales, Pilar ; Santiago-Mauricio, María Guadalupe ; Guzmán-Delgado, Nancy Elena ; Vargas-Villarreal, Javier ; Lozano-Garza, Gerardo ; Viveros-Valdez, Ezequiel ; Ortiz-López, Rocío ; Morán-Martínez, Javier ; Gandolfi, A Jay. / Induction of virulence factors, apoptosis, and cytokines in precision-cut hamster liver slices infected with Entamoeba histolytica. In: Experimental Parasitology. 2012 ; Vol. 132, No. 4. pp. 424-433.
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AU - Lozano-Garza, Gerardo

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