Inflammatory and hemodynamic changes in the cerebral microcirculation of aged rats after global cerebral ischemia and reperfusion

Leslie S Ritter, Janet L Funk, Lori Schenkel, Alicia Tipton, Kate Downey, Jonathan Wilson, Bruce M Coull, Paul McDonagh

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Effects of aging on inflammation and blood flow in the brain are unclear. Young (three to six months) and aged (19-22 months) male Brown Norway Fisher rats were used to compare (i) leukocyte function in nonischemic conditions and (ii) leukocyte function and hemodynamic changes after ischemia-reperfusion (I-R). In nonischemic studies, polymorphonuclear (PMN) CD11b expression and reactive oxygen species (ROS) production were measured with flow cytometry and PMN chemotaxis was measured with a Boyden chamber (+/-fMLP). In I-R studies, ischemia was induced by bilateral carotid artery occlusion and hypotension (20 minutes). During early reperfusion (30 minutes), leukocyte adhesion and rolling and blood-shear rates were measured using fluorescence microscopy. During late reperfusion (48 hours), mortality, neurological function, and leukocyte infiltration were measured. Stimulated PMN chemotaxis was increased in nonischemic aged rats (p < 0.05). In early reperfusion, there was a significant increase in leukocyte rolling and adhesion in the cerebral microcirculation and a significant decrease in shear rate in aged rats, compared to the young (p < 0.05). During late reperfusion, neurologic function was worse in aged vs. young rats (p < 0.05). These findings suggest that increased intravascular PMN adhesion and vascular dysfunction may contribute to poor neurologic outcome after cerebral I-R in the aged brain.

Original languageEnglish (US)
Pages (from-to)297-310
Number of pages14
JournalMicrocirculation
Volume15
Issue number4
DOIs
StatePublished - May 2008

Fingerprint

Microcirculation
Brain Ischemia
Reperfusion
Hemodynamics
Leukocyte Rolling
Leukocytes
Ischemia
Chemotaxis
Nervous System
Brain
Carotid Arteries
Fluorescence Microscopy
Hypotension
Blood Vessels
Reactive Oxygen Species
Flow Cytometry
Inflammation
Mortality

Keywords

  • Cerebral microcirculation
  • Ischemia-reperfusion injury
  • Leukocyte
  • Stroke

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Cardiology and Cardiovascular Medicine

Cite this

Inflammatory and hemodynamic changes in the cerebral microcirculation of aged rats after global cerebral ischemia and reperfusion. / Ritter, Leslie S; Funk, Janet L; Schenkel, Lori; Tipton, Alicia; Downey, Kate; Wilson, Jonathan; Coull, Bruce M; McDonagh, Paul.

In: Microcirculation, Vol. 15, No. 4, 05.2008, p. 297-310.

Research output: Contribution to journalArticle

Ritter, Leslie S ; Funk, Janet L ; Schenkel, Lori ; Tipton, Alicia ; Downey, Kate ; Wilson, Jonathan ; Coull, Bruce M ; McDonagh, Paul. / Inflammatory and hemodynamic changes in the cerebral microcirculation of aged rats after global cerebral ischemia and reperfusion. In: Microcirculation. 2008 ; Vol. 15, No. 4. pp. 297-310.
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