Influence of 1,25-Di hydroxy vitamin D3 on Cultured Osteogenic Sarcoma Cells: Correlation with the 1,25-Dihydroxyvitamin D3 Receptor

Shigeharu Dokoh, Carol A. Donaldson, Mark R. Haussler

Research output: Contribution to journalArticlepeer-review

135 Scopus citations

Abstract

We have characterized the effects of the steroid hormone 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] on a series of rat osteogenic sarcoma cell lines of increasing osteoblastic-like nature (ROS 24/1, ROS 2/3, and ROS 17/2.8). When these cells were grown in monolayer culture in the presence of 10 nM 1,25-(OHfeDa, there was a dramatic and selective inhibition of proliferation in the ROS 17/2.8 line. Similar concentrations of other vitamin D metabolites did not elicit this effect. Furthermore, the aggregated cuboidal ROS 17/2.8 cells showed a marked change after 6 days of treatment with 10 nM 1,25-(OH)2D3 to an apparently less transformed spindle-like morphology. In contrast, ROS 2/3 displayed only a slight morphological alteration, and ROS 24/1 was unchanged by treatment with 1,25-{OH)2D3. Anchorage-independent growth studies performed in soft agar indicated that 1,25-(OH)2D3 inhibited colony formation to the greatest degree in ROS 17/2.8, with a lesser effect in ROS 2/3. Based upon analyses by sucrose gradient centrifugation, DNA cellulose chromatography, and saturation of specific binding, the level of the 1,25-(OH)2D3 receptor was quantitated in these cells. ROS 17/2.8 cells possess 18,000 copies of the receptor per cell, while ROS 2/3 contains only 500 binding sites per cell, and no detectable high-affinity 1,25-(OH)2D3 receptor is found in ROS 24/1. The receptor in ROS cells is indistinguishable from other mammalian 1,25-(OH)2D3 receptors in that it is a DNA-binding protein that sediments on sucrose gradients at 3.3S, and specifically binds the hormone with high affinity (Kd = 2 to 3 x 10-11 M). Since the biological responses of these three cell lines to 1,25-(OH)2D3 exhibit a strong correlation with the respective number of receptor molecules per cell, we propose that the actions of this hormone are mediated by the specific 1,25-{OH)2D3 receptor.

Original languageEnglish (US)
Pages (from-to)2103-2109
Number of pages7
JournalCancer Research
Volume44
Issue number5
StatePublished - May 1 1984

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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