Influence of estrogen on renal vitamin D hydroxylases and serum 1alpha,25-(OH)2D3 in chicks.

J. W. Pike, E. Spanos, K. W. Colston, I. MacIntyre, Mark R Haussler

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Abstract

The influence of estrogen on the metabolism of 25-hydroxyvitamin D3 was studied in 2- to 5-wk-old chicks. Single injections of at least 500 microgram diethylstibestrol (DES) increased the conversion of 25-hydroxyvitamin D3 to 1alpha,25-dihydroxyvitamin D3 (1alpha,25-(OH)2D3) and suppressed the production of 24,25-dihydroxyvitamin D3 in chick kidney homogenates. Acute (one 5-mg) injections of testosterone or progesterone did not enhance the 25-hydroxyvitamin D3-1alpha-hydroxylase, indicating specificity. However, chronic pretreatment with DES appeared to allow the potentiation of previously unstimulatory steroids such as progesterone and testosterone. In addition, the hormonal metabolite of vitamin D3, 1alpha,25-(OH)2D3, was measured in 4- to 6-wk chick plasma after steroid treatment. Greater than 1 mg DES per day for 5 days was necessary to enhance the circulating level of 1alpha,25-(OH)2D3; testosterone alone had no effect. This elevation was rapid, occurring within 12--24 h after injection. These data suggest that estrogen (as evidenced by DES treatment) is a modulator of vitamin D metabolism along with other known regulators such as parathyroid hormone, phosphate, and 1alpha,25-(OH)2D3. The mechanism of the regulation is as yet unknown.

Original languageEnglish (US)
JournalThe American journal of physiology
Volume235
Issue number3
StatePublished - Sep 1978
Externally publishedYes

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Calcifediol
Mixed Function Oxygenases
Vitamin D
Testosterone
Estrogens
Kidney
Injections
Progesterone
24,25-Dihydroxyvitamin D 3
Serum
Steroids
Cholecalciferol
Parathyroid Hormone
Phosphates
Therapeutics

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Influence of estrogen on renal vitamin D hydroxylases and serum 1alpha,25-(OH)2D3 in chicks. / Pike, J. W.; Spanos, E.; Colston, K. W.; MacIntyre, I.; Haussler, Mark R.

In: The American journal of physiology, Vol. 235, No. 3, 09.1978.

Research output: Contribution to journalArticle

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AU - Haussler, Mark R

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