Abstract
Rhodopsin in native rod membranes and incorporated into egg phosphatidylcholine (egg PC) vesicles was studied at pH 5 and 7 at 28°C. Rhodopsin function, as monitored by the formation of metarhodopsin II (MII) from metarhodopsin I (MI) following an actinic flash, was found to be largely blocked in egg PC vesicles at pH 7. When the pH was lowered to 5, however, rhodopsin showed essentially equal activity in both native and egg PC membranes. This activity exceeded that found for rhodopsin in native membranes at pH 7. Phospholipid composition is thus shown to directly affect the MI ⇌ MII equilibrium, which in turn is linked to visual function.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1028-1034 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 169 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jun 29 1990 |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
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Influence of pH on the MI-MII equilibrium of rhodopsin in recombinant membranes. / Gibson, Nicholas J.; Brown, Michael F.
In: Biochemical and Biophysical Research Communications, Vol. 169, No. 3, 29.06.1990, p. 1028-1034.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Influence of pH on the MI-MII equilibrium of rhodopsin in recombinant membranes
AU - Gibson, Nicholas J.
AU - Brown, Michael F
PY - 1990/6/29
Y1 - 1990/6/29
N2 - Rhodopsin in native rod membranes and incorporated into egg phosphatidylcholine (egg PC) vesicles was studied at pH 5 and 7 at 28°C. Rhodopsin function, as monitored by the formation of metarhodopsin II (MII) from metarhodopsin I (MI) following an actinic flash, was found to be largely blocked in egg PC vesicles at pH 7. When the pH was lowered to 5, however, rhodopsin showed essentially equal activity in both native and egg PC membranes. This activity exceeded that found for rhodopsin in native membranes at pH 7. Phospholipid composition is thus shown to directly affect the MI ⇌ MII equilibrium, which in turn is linked to visual function.
AB - Rhodopsin in native rod membranes and incorporated into egg phosphatidylcholine (egg PC) vesicles was studied at pH 5 and 7 at 28°C. Rhodopsin function, as monitored by the formation of metarhodopsin II (MII) from metarhodopsin I (MI) following an actinic flash, was found to be largely blocked in egg PC vesicles at pH 7. When the pH was lowered to 5, however, rhodopsin showed essentially equal activity in both native and egg PC membranes. This activity exceeded that found for rhodopsin in native membranes at pH 7. Phospholipid composition is thus shown to directly affect the MI ⇌ MII equilibrium, which in turn is linked to visual function.
UR - http://www.scopus.com/inward/record.url?scp=0025301617&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025301617&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(90)91997-7
DO - 10.1016/0006-291X(90)91997-7
M3 - Article
C2 - 2363712
AN - SCOPUS:0025301617
VL - 169
SP - 1028
EP - 1034
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -