Influence of temperature and unstirred layers on the kinetics of glycine transport in isolated gills of Mytilus californianus

Stephen H. Wright, Steven A. Becker, Grover C. Stephens

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Abstract

The effect of temperature on the kinetics of glycine influx in isolated gills of Mytilus californianus was studied. Increases in temperature resulted in significant increases in the J imar for glycine influx over the range 7–23° C (Q10 = 5). Concomitant with this increase in J imar was an increase in experimentally determined Kt, from 23 μM to 134 μM. The relationship between changes in J imar and measured Kt was adequately described by an equation that takes into account the influence of unstirred layers on the kinetics of carriermediated transport. Use of this equation indicated that the changes in measured Kt could be explained by the presence of an unstirred layer approximately 400 μm thick over the transporting surfaces(s) of the isolated gill, and that the “true” Kt of the transport process is 1 μM. The very low values Kt for amino acid uptake measured in intact mussels (1–3 μM) are revealed to be the product of transport processes of high affinity for substrate and a perfusion system which effectively minimizes the influence of unstirred layers, thereby permitting efficient utilization of substrate at the low concentrations characteristic of these animals' environment.

Original languageEnglish (US)
Pages (from-to)27-35
Number of pages9
JournalJournal of Experimental Zoology
Volume214
Issue number1
DOIs
StatePublished - Oct 1980
Externally publishedYes

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ASJC Scopus subject areas

  • Animal Science and Zoology

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