Influences of membrane curvature in lipid hexagonal phases studied by deuterium NMR spectroscopy

Robin L. Thurmond, Göran Lindblom, Michael F. Brown

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

The presence of reversed hexagonal phase, HII, favoring lipids in membranes has been proposed to be significant in various biological processes. Therefore an understanding of the HII phase and the transition from the lamellar to hexagonal phase is of importance. We have applied deuterium NMR spectroscopy to study the bilayer and reversed hexagonal phases of 1-perdeuteriopalmitoyl-2-linoleoyl-sn-glycero-3-phosphoethanolamine. The difference in packing between the HII and Lα phases leads to smaller segmental order parameters in the former case. Since the order profiles are sensitive to the geometry of the aggregates, they can be used to extract structural information about the phases. We present a new means of calculating the radius of curvature, R1, for the HII phase from 2H NMR data. This method gives a value of R1 = 18.1 Å, which is in agreement with current understanding of the structure of the HII phase and with x-ray diffraction data.

Original languageEnglish (US)
Pages (from-to)1231-1238
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume173
Issue number3
DOIs
StatePublished - Dec 31 1990

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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