Ingested human insulin inhibits the mosquito NF-κB-dependent immune response to Plasmodium falciparum

Nazzy Pakpour, Vanessa Corby-Harris, Gabriel P. Green, Hannah M. Smithers, Kong W. Cheung, Michael A Riehle, Shirley Luckhart

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We showed previously that ingested human insulin activates the insulin/IGF-1 signaling pathway in Anopheles stephensi and increases the susceptibility of these mosquitoes to Plasmodium falciparum. In other organisms, insulin can alter immune responsiveness through regulation of NF-κB transcription factors, critical elements for innate immunity that are also central to mosquito immunity. We show here that insulin signaling decreased expression of NF-κB-regulated immune genes in mosquito cells stimulated with either bacterial or malarial soluble products. Further, human insulin suppressed mosquito immunity through sustained phosphatidylinositol 3-kinase activation, since inhibition of this pathway led to decreased parasite development in the mosquito. Together, these data demonstrate that activation of the insulin/IGF-1 signaling pathway by ingested human insulin can alter NF-κB-dependent immunity, and ultimately the susceptibility, of mosquitoes to P. falciparum.

Original languageEnglish (US)
Pages (from-to)2141-2149
Number of pages9
JournalInfection and Immunity
Volume80
Issue number6
DOIs
StatePublished - Jan 1 2012

Fingerprint

Plasmodium falciparum
Culicidae
Insulin
Immunity
Insulin-Like Growth Factor I
Phosphatidylinositol 3-Kinase
Anopheles
Innate Immunity
Parasites
Transcription Factors
Genes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Pakpour, N., Corby-Harris, V., Green, G. P., Smithers, H. M., Cheung, K. W., Riehle, M. A., & Luckhart, S. (2012). Ingested human insulin inhibits the mosquito NF-κB-dependent immune response to Plasmodium falciparum. Infection and Immunity, 80(6), 2141-2149. https://doi.org/10.1128/IAI.00024-12

Ingested human insulin inhibits the mosquito NF-κB-dependent immune response to Plasmodium falciparum. / Pakpour, Nazzy; Corby-Harris, Vanessa; Green, Gabriel P.; Smithers, Hannah M.; Cheung, Kong W.; Riehle, Michael A; Luckhart, Shirley.

In: Infection and Immunity, Vol. 80, No. 6, 01.01.2012, p. 2141-2149.

Research output: Contribution to journalArticle

Pakpour, N, Corby-Harris, V, Green, GP, Smithers, HM, Cheung, KW, Riehle, MA & Luckhart, S 2012, 'Ingested human insulin inhibits the mosquito NF-κB-dependent immune response to Plasmodium falciparum', Infection and Immunity, vol. 80, no. 6, pp. 2141-2149. https://doi.org/10.1128/IAI.00024-12
Pakpour, Nazzy ; Corby-Harris, Vanessa ; Green, Gabriel P. ; Smithers, Hannah M. ; Cheung, Kong W. ; Riehle, Michael A ; Luckhart, Shirley. / Ingested human insulin inhibits the mosquito NF-κB-dependent immune response to Plasmodium falciparum. In: Infection and Immunity. 2012 ; Vol. 80, No. 6. pp. 2141-2149.
@article{abcaba450cfb49558e4d975852f60e6e,
title = "Ingested human insulin inhibits the mosquito NF-κB-dependent immune response to Plasmodium falciparum",
abstract = "We showed previously that ingested human insulin activates the insulin/IGF-1 signaling pathway in Anopheles stephensi and increases the susceptibility of these mosquitoes to Plasmodium falciparum. In other organisms, insulin can alter immune responsiveness through regulation of NF-κB transcription factors, critical elements for innate immunity that are also central to mosquito immunity. We show here that insulin signaling decreased expression of NF-κB-regulated immune genes in mosquito cells stimulated with either bacterial or malarial soluble products. Further, human insulin suppressed mosquito immunity through sustained phosphatidylinositol 3-kinase activation, since inhibition of this pathway led to decreased parasite development in the mosquito. Together, these data demonstrate that activation of the insulin/IGF-1 signaling pathway by ingested human insulin can alter NF-κB-dependent immunity, and ultimately the susceptibility, of mosquitoes to P. falciparum.",
author = "Nazzy Pakpour and Vanessa Corby-Harris and Green, {Gabriel P.} and Smithers, {Hannah M.} and Cheung, {Kong W.} and Riehle, {Michael A} and Shirley Luckhart",
year = "2012",
month = "1",
day = "1",
doi = "10.1128/IAI.00024-12",
language = "English (US)",
volume = "80",
pages = "2141--2149",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "6",

}

TY - JOUR

T1 - Ingested human insulin inhibits the mosquito NF-κB-dependent immune response to Plasmodium falciparum

AU - Pakpour, Nazzy

AU - Corby-Harris, Vanessa

AU - Green, Gabriel P.

AU - Smithers, Hannah M.

AU - Cheung, Kong W.

AU - Riehle, Michael A

AU - Luckhart, Shirley

PY - 2012/1/1

Y1 - 2012/1/1

N2 - We showed previously that ingested human insulin activates the insulin/IGF-1 signaling pathway in Anopheles stephensi and increases the susceptibility of these mosquitoes to Plasmodium falciparum. In other organisms, insulin can alter immune responsiveness through regulation of NF-κB transcription factors, critical elements for innate immunity that are also central to mosquito immunity. We show here that insulin signaling decreased expression of NF-κB-regulated immune genes in mosquito cells stimulated with either bacterial or malarial soluble products. Further, human insulin suppressed mosquito immunity through sustained phosphatidylinositol 3-kinase activation, since inhibition of this pathway led to decreased parasite development in the mosquito. Together, these data demonstrate that activation of the insulin/IGF-1 signaling pathway by ingested human insulin can alter NF-κB-dependent immunity, and ultimately the susceptibility, of mosquitoes to P. falciparum.

AB - We showed previously that ingested human insulin activates the insulin/IGF-1 signaling pathway in Anopheles stephensi and increases the susceptibility of these mosquitoes to Plasmodium falciparum. In other organisms, insulin can alter immune responsiveness through regulation of NF-κB transcription factors, critical elements for innate immunity that are also central to mosquito immunity. We show here that insulin signaling decreased expression of NF-κB-regulated immune genes in mosquito cells stimulated with either bacterial or malarial soluble products. Further, human insulin suppressed mosquito immunity through sustained phosphatidylinositol 3-kinase activation, since inhibition of this pathway led to decreased parasite development in the mosquito. Together, these data demonstrate that activation of the insulin/IGF-1 signaling pathway by ingested human insulin can alter NF-κB-dependent immunity, and ultimately the susceptibility, of mosquitoes to P. falciparum.

UR - http://www.scopus.com/inward/record.url?scp=85047687055&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047687055&partnerID=8YFLogxK

U2 - 10.1128/IAI.00024-12

DO - 10.1128/IAI.00024-12

M3 - Article

C2 - 22473605

AN - SCOPUS:85047687055

VL - 80

SP - 2141

EP - 2149

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 6

ER -