Inhalation exposure to JP-8 jet fuel alters pulmonary function and substance P levels in Fischer 344 rats

J. Pfaff, K. Parton, Robert Clark Lantz, H. Chen, A. M. Hays, M. L. Witten

Research output: Contribution to journalArticle

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Abstract

In a simulated military flightline exposure protocol, Fischer 344 rats (F344) were used to investigate the pulmonary effects of JP-8 jet fuel inhalation. Exposures were nose only and for 1 h daily. Groups were exposed for 7 days (7D) or 28 days (28D). Each exposure group had a matched longitudinal control group (LC7 and LC28). Exposure concentrations of 520 mg m-3 caused an increase in dynamic compliance after 7 days of exposure, but compliance changes were not seen with continued exposure (28D, 495 mg m-3). Pulmonary resistance was increased in both 7- and 28-day JP-8 exposed groups. Changes in pulmonary function were accompanied by a decrease in substance P concentrations from the bronchoalveolar lavage fluid (BALF). No significant change was observed in BALF levels of 6-keto-PGF(1α) the stable metabolite of prostacyclin, which is a marker of endothelial cell function. The JP-8-exposed rats gained significantly less weight during the study period than the LC7 and LC28 groups, and the lungs of the 7D group were heavier by wet lung/body weight ratio ((Wt(L)/Wt(B)). Alveolar clearance of technetium-labelled diethylenetriamine pentaacetate ([99mTc]DTPA) was increased in jet fuel-exposed groups. Light microscopy showed no pathological evidence of lung injury. Recovery from the early pulmonary effects of JP-8 inhalation occurred with continued exposure, as seen by recovery of pulmonary compliance and Wt(L)/Wt(B).

Original languageEnglish (US)
Pages (from-to)249-256
Number of pages8
JournalJournal of Applied Toxicology
Volume15
Issue number4
DOIs
StatePublished - 1995

Fingerprint

Inhalation Exposure
Inbred F344 Rats
Substance P
Rats
Lung
Longitudinal control
Bronchoalveolar Lavage Fluid
Recovery
Pentetic Acid
Fluids
Jet fuel
Technetium
Inhalation
Endothelial cells
Compliance
Prostaglandins F
Epoprostenol
Metabolites
Optical microscopy
Lung Compliance

Keywords

  • 6-Keto-prostaglandin F(1α)
  • Diethylenetriamine pentaacetate ([(M)Tc]DTPA)
  • Dynamic compliance
  • Hydrocarbon inhalation
  • Jet fuel
  • JP-8
  • Prostacyclin
  • Pulmonary resistance
  • Substance P

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Inhalation exposure to JP-8 jet fuel alters pulmonary function and substance P levels in Fischer 344 rats. / Pfaff, J.; Parton, K.; Lantz, Robert Clark; Chen, H.; Hays, A. M.; Witten, M. L.

In: Journal of Applied Toxicology, Vol. 15, No. 4, 1995, p. 249-256.

Research output: Contribution to journalArticle

Pfaff, J. ; Parton, K. ; Lantz, Robert Clark ; Chen, H. ; Hays, A. M. ; Witten, M. L. / Inhalation exposure to JP-8 jet fuel alters pulmonary function and substance P levels in Fischer 344 rats. In: Journal of Applied Toxicology. 1995 ; Vol. 15, No. 4. pp. 249-256.
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AU - Witten, M. L.

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