Inheritance of resistance to crown gall in Pisum sativum

Steven L. Robbs, Martha C Hawes, Hao Jan Lin, Steven G. Pueppke, Laura Y. Smith

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We screened a total of 1365 pea (Pisum sativum) lines for response to inoculation with Agrobacterium tumefaciens, strain B6, and characterized resistance in one cultivar, Sweet Snap. Sweet Snap seedlings were highly resistant to tumorigenesis under most conditions. Resistance was overcome at inoculum concentrations of greater than 109 bacteria per milliliter. At such high concentrations, very small tumors developed on Sweet Snap in response to four wide-host-range Agrobacterium strains, but tumors on other cultivars were two- to sevenfold larger than those that formed on Sweet Snap. The hypervirulent strain A281 induced larger tumors on Sweet Snap than did other Agrobacterium strains, but tumors on other genotypes were more than 100% larger than those on Sweet Snap. Physiological experiments suggested that tumorigenesis in Sweet Snap is not blocked in early stages of infection, and genetic analysis indicated that inheritance of resistance to crown gall is a quantitative trait. In addition to the observed resistance in Sweet Snap, three 'supersusceptible' genotypes, which developed very large tumors, also were identified.

Original languageEnglish (US)
Pages (from-to)52-57
Number of pages6
JournalPlant Physiology
Volume95
Issue number1
StatePublished - Jan 1991

Fingerprint

Plant Tumors
crown galls
Peas
Pisum sativum
inheritance (genetics)
Agrobacterium
Neoplasms
neoplasms
Carcinogenesis
Genotype
Agrobacterium tumefaciens
carcinogenesis
Host Specificity
Seedlings
genotype
cultivars
Bacteria
Agrobacterium radiobacter
quantitative traits
host range

ASJC Scopus subject areas

  • Plant Science

Cite this

Robbs, S. L., Hawes, M. C., Lin, H. J., Pueppke, S. G., & Smith, L. Y. (1991). Inheritance of resistance to crown gall in Pisum sativum. Plant Physiology, 95(1), 52-57.

Inheritance of resistance to crown gall in Pisum sativum. / Robbs, Steven L.; Hawes, Martha C; Lin, Hao Jan; Pueppke, Steven G.; Smith, Laura Y.

In: Plant Physiology, Vol. 95, No. 1, 01.1991, p. 52-57.

Research output: Contribution to journalArticle

Robbs, SL, Hawes, MC, Lin, HJ, Pueppke, SG & Smith, LY 1991, 'Inheritance of resistance to crown gall in Pisum sativum', Plant Physiology, vol. 95, no. 1, pp. 52-57.
Robbs SL, Hawes MC, Lin HJ, Pueppke SG, Smith LY. Inheritance of resistance to crown gall in Pisum sativum. Plant Physiology. 1991 Jan;95(1):52-57.
Robbs, Steven L. ; Hawes, Martha C ; Lin, Hao Jan ; Pueppke, Steven G. ; Smith, Laura Y. / Inheritance of resistance to crown gall in Pisum sativum. In: Plant Physiology. 1991 ; Vol. 95, No. 1. pp. 52-57.
@article{83c7845d51a24556870a95cea8c014a1,
title = "Inheritance of resistance to crown gall in Pisum sativum",
abstract = "We screened a total of 1365 pea (Pisum sativum) lines for response to inoculation with Agrobacterium tumefaciens, strain B6, and characterized resistance in one cultivar, Sweet Snap. Sweet Snap seedlings were highly resistant to tumorigenesis under most conditions. Resistance was overcome at inoculum concentrations of greater than 109 bacteria per milliliter. At such high concentrations, very small tumors developed on Sweet Snap in response to four wide-host-range Agrobacterium strains, but tumors on other cultivars were two- to sevenfold larger than those that formed on Sweet Snap. The hypervirulent strain A281 induced larger tumors on Sweet Snap than did other Agrobacterium strains, but tumors on other genotypes were more than 100{\%} larger than those on Sweet Snap. Physiological experiments suggested that tumorigenesis in Sweet Snap is not blocked in early stages of infection, and genetic analysis indicated that inheritance of resistance to crown gall is a quantitative trait. In addition to the observed resistance in Sweet Snap, three 'supersusceptible' genotypes, which developed very large tumors, also were identified.",
author = "Robbs, {Steven L.} and Hawes, {Martha C} and Lin, {Hao Jan} and Pueppke, {Steven G.} and Smith, {Laura Y.}",
year = "1991",
month = "1",
language = "English (US)",
volume = "95",
pages = "52--57",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "1",

}

TY - JOUR

T1 - Inheritance of resistance to crown gall in Pisum sativum

AU - Robbs, Steven L.

AU - Hawes, Martha C

AU - Lin, Hao Jan

AU - Pueppke, Steven G.

AU - Smith, Laura Y.

PY - 1991/1

Y1 - 1991/1

N2 - We screened a total of 1365 pea (Pisum sativum) lines for response to inoculation with Agrobacterium tumefaciens, strain B6, and characterized resistance in one cultivar, Sweet Snap. Sweet Snap seedlings were highly resistant to tumorigenesis under most conditions. Resistance was overcome at inoculum concentrations of greater than 109 bacteria per milliliter. At such high concentrations, very small tumors developed on Sweet Snap in response to four wide-host-range Agrobacterium strains, but tumors on other cultivars were two- to sevenfold larger than those that formed on Sweet Snap. The hypervirulent strain A281 induced larger tumors on Sweet Snap than did other Agrobacterium strains, but tumors on other genotypes were more than 100% larger than those on Sweet Snap. Physiological experiments suggested that tumorigenesis in Sweet Snap is not blocked in early stages of infection, and genetic analysis indicated that inheritance of resistance to crown gall is a quantitative trait. In addition to the observed resistance in Sweet Snap, three 'supersusceptible' genotypes, which developed very large tumors, also were identified.

AB - We screened a total of 1365 pea (Pisum sativum) lines for response to inoculation with Agrobacterium tumefaciens, strain B6, and characterized resistance in one cultivar, Sweet Snap. Sweet Snap seedlings were highly resistant to tumorigenesis under most conditions. Resistance was overcome at inoculum concentrations of greater than 109 bacteria per milliliter. At such high concentrations, very small tumors developed on Sweet Snap in response to four wide-host-range Agrobacterium strains, but tumors on other cultivars were two- to sevenfold larger than those that formed on Sweet Snap. The hypervirulent strain A281 induced larger tumors on Sweet Snap than did other Agrobacterium strains, but tumors on other genotypes were more than 100% larger than those on Sweet Snap. Physiological experiments suggested that tumorigenesis in Sweet Snap is not blocked in early stages of infection, and genetic analysis indicated that inheritance of resistance to crown gall is a quantitative trait. In addition to the observed resistance in Sweet Snap, three 'supersusceptible' genotypes, which developed very large tumors, also were identified.

UR - http://www.scopus.com/inward/record.url?scp=0002849775&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0002849775&partnerID=8YFLogxK

M3 - Article

C2 - 16667980

AN - SCOPUS:0002849775

VL - 95

SP - 52

EP - 57

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 1

ER -