Inhibition of CXCR4-tropic HIV-1 infection by lipopolysaccharide: Evidence of different mechanisms in macrophages and T lymphocytes

Alessia Verani, Francesca Sironi, Antonio G. Siccardi, Paolo Lusso, Donata Vercelli

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Bacterial LPS protects primary human macrophages from infection by CCR5-tropic HIV-1 isolates through the release of the CC chemokines RANTES and macrophage inflammatory protein-1α and -1β. Here, we show that LPS also suppresses infection of macrophages by CXCR4-tropic HIV-1 isolates. A marked down-regulation of both CD4 and CXCR4 expression was associated with this effect. Furthermore, a soluble factor(s) released by macrophages upon LPS treatment inhibited infection with CXCR4-tropic HIV-1 isolate viruses in both macrophages and T lymphocytes. Infection of both cell types appeared to be blocked at the level of viral entry and was independent of stromal cell-derived factor-1, the only known natural ligand of CXCR4. Moreover, the suppressive effect of LPS was unrelated to the release of IFN-α and -β, macrophage-derived chemokine, leukemia inhibitory factor, or TNF-α. These results suggest the existence of potent HIV-1 inhibitory factor(s), uncharacterized to date, released by activated cells of the mononuclear phagocytic system.

Original languageEnglish (US)
Pages (from-to)6388-6395
Number of pages8
JournalJournal of Immunology
Volume168
Issue number12
StatePublished - Jun 15 2002
Externally publishedYes

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HIV Infections
Lipopolysaccharides
HIV-1
Macrophages
T-Lymphocytes
Infection
Chemokine CCL22
Macrophage Inflammatory Proteins
Leukemia Inhibitory Factor
Chemokine CXCL12
Chemokine CCL5
CC Chemokines
Phagocytes
Down-Regulation
Ligands
Viruses

ASJC Scopus subject areas

  • Immunology

Cite this

Inhibition of CXCR4-tropic HIV-1 infection by lipopolysaccharide : Evidence of different mechanisms in macrophages and T lymphocytes. / Verani, Alessia; Sironi, Francesca; Siccardi, Antonio G.; Lusso, Paolo; Vercelli, Donata.

In: Journal of Immunology, Vol. 168, No. 12, 15.06.2002, p. 6388-6395.

Research output: Contribution to journalArticle

Verani, Alessia ; Sironi, Francesca ; Siccardi, Antonio G. ; Lusso, Paolo ; Vercelli, Donata. / Inhibition of CXCR4-tropic HIV-1 infection by lipopolysaccharide : Evidence of different mechanisms in macrophages and T lymphocytes. In: Journal of Immunology. 2002 ; Vol. 168, No. 12. pp. 6388-6395.
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