Inhibition of endothelial regeneration by type-beta transforming growth factor from platelets

Ronald L Heimark, Daniel R. Twardzik, Stephen M. Schwartz

Research output: Contribution to journalArticle

326 Citations (Scopus)

Abstract

Damage to the vessel wall is a signal for endothelial migration and replication and for platelet release at the site of injury. Addition of transforming growth factor-beta (TGF-β) purified from platelets to growing aortic endothelial cells inhibited [3H]thymidine incorporation in a concentration-dependent manner. A transient inhibition of DNA synthesis was also observed in response to wounding; cell migration and replication are inhibited during the first 24 hours after wounding. By 48 hours after wounding both TGF-β-treated and -untreated cultures showed similar responses. Flow microfluorimetric analysis of cell cycle distribution indicated that after 24 hours of exposure to TGF-β the cells were blocked from entering S phase, and the fraction of cells in G1 was increased. The inhibition of the initiation of regeneration by TGF-β could allow time for recruitment of smooth muscle cells into the site of injury by other platelet components.

Original languageEnglish (US)
Pages (from-to)1078-1080
Number of pages3
JournalScience
Volume233
Issue number4768
StatePublished - 1986
Externally publishedYes

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Transforming Growth Factor beta
Regeneration
Blood Platelets
Wounds and Injuries
S Phase
Thymidine
Smooth Muscle Myocytes
Cell Movement
Cell Cycle
Endothelial Cells
DNA

ASJC Scopus subject areas

  • General

Cite this

Inhibition of endothelial regeneration by type-beta transforming growth factor from platelets. / Heimark, Ronald L; Twardzik, Daniel R.; Schwartz, Stephen M.

In: Science, Vol. 233, No. 4768, 1986, p. 1078-1080.

Research output: Contribution to journalArticle

Heimark, RL, Twardzik, DR & Schwartz, SM 1986, 'Inhibition of endothelial regeneration by type-beta transforming growth factor from platelets', Science, vol. 233, no. 4768, pp. 1078-1080.
Heimark, Ronald L ; Twardzik, Daniel R. ; Schwartz, Stephen M. / Inhibition of endothelial regeneration by type-beta transforming growth factor from platelets. In: Science. 1986 ; Vol. 233, No. 4768. pp. 1078-1080.
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