Inhibition of endothelial regeneration by type-beta transforming growth factor from platelets

Ronald L. Heimark, Daniel R. Twardzik, Stephen M. Schwartz

Research output: Contribution to journalArticle

327 Scopus citations

Abstract

Damage to the vessel wall is a signal for endothelial migration and replication and for platelet release at the site of injury. Addition of transforming growth factor-beta (TGF-β) purified from platelets to growing aortic endothelial cells inhibited [3H]thymidine incorporation in a concentration-dependent manner. A transient inhibition of DNA synthesis was also observed in response to wounding; cell migration and replication are inhibited during the first 24 hours after wounding. By 48 hours after wounding both TGF-β-treated and -untreated cultures showed similar responses. Flow microfluorimetric analysis of cell cycle distribution indicated that after 24 hours of exposure to TGF-β the cells were blocked from entering S phase, and the fraction of cells in G1 was increased. The inhibition of the initiation of regeneration by TGF-β could allow time for recruitment of smooth muscle cells into the site of injury by other platelet components.

Original languageEnglish (US)
Pages (from-to)1078-1080
Number of pages3
JournalScience
Volume233
Issue number4768
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

ASJC Scopus subject areas

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