Inhibition of glycolysis by 2-DG increases [Ca2+](i) in pulmonary arterial smooth muscle cells

R. T. Bright, C. G. Salvaterra, L. J. Rubin, Jason Yuan

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Inhibition of glycolysis depolarizes single pulmonary arterial smooth muscle cells (PASMC) and potentiates hypoxic pulmonary vasoconstriction (HPV) in isolated perfused rat lungs. Whether glycolytic inhibition causes an increase in the intracellular Ca2+ concentration ([Ca2+](i)) in PASMC was determined in this study. [Ca2+](i) was measured in primary cultured rat PASMC using the Ca2+-sensitive fluorescent indicator, fura 2, and quantitative fluorescence microscopy. Extracellular application of the glycolytic inhibitor, 2-deoxy-D-glucose (2-DG), significantly and reversibly increased [Ca2+](i) in PASMC. Removal of extracellular Ca2+ and application of the Ca2+ channel blocker, verapamil (10 μM), attenuated, but did not eliminate, the 2-DG-induced rise in [Ca2+](i). In the absence of extracellular Ca2+, however, depletion of inositol triphosphate- sensitive intracellular Ca2+ stores by 10 μM cyclopiazonic acid (CPA) completely abolished the 2-DG-induced increase in [Ca2+](i). The data suggest that 2-DG-induced increases in [Ca2+](i) result from both Ca2+ influx through the verapamil-sensitive voltage-gated Ca2+ channels and Ca2+ release from the CPA-sensitive intracellular Ca2+ stores.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume269
Issue number2 13-2
StatePublished - 1995
Externally publishedYes

Fingerprint

Deoxyglucose
glycolysis
Glycolysis
smooth muscle
myocytes
Smooth Muscle Myocytes
lungs
calcium
Lung
glucose
Verapamil
Fura-2
cyclopiazonic acid
Inositol
Vasoconstriction
Fluorescence Microscopy
verapamil
vasoconstriction
rats
fluorescence microscopy

Keywords

  • 2-deoxy-D-glucose
  • intracellular calcium

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology
  • Agricultural and Biological Sciences(all)

Cite this

Inhibition of glycolysis by 2-DG increases [Ca2+](i) in pulmonary arterial smooth muscle cells. / Bright, R. T.; Salvaterra, C. G.; Rubin, L. J.; Yuan, Jason.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 269, No. 2 13-2, 1995.

Research output: Contribution to journalArticle

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