Inhibition of nuclear import and alteration of nuclear pore complex composition by rhinovirus

Kurt E Gustin, Peter Sarnow

Research output: Contribution to journalArticle

131 Citations (Scopus)

Abstract

Nucleocytoplasmic trafficking pathways and the status of nuclear pore complex (NPC) components were examined in cells infected with rhinovirus type 14. A variety of shuttling and nonshuttling nuclear proteins, using multiple nuclear import pathways, accumulated in the cytoplasm of cells infected with rhinovirus. An in vitro nuclear import assay with semipermeabilized infected cells confirmed that nuclear import was inhibited and that docking of nuclear import receptor-cargo complexes at the cytoplasmic face of the NPC was prevented in rhinovirus -infected cells. The relocation of cellular proteins and inhibition of nuclear import correlated with the degradation of two NPC components, Nup153 and p62. The degradation of Nup153 and p62 was not due to induction of apoptosis, because p62 was not proteolyzed in apoptotic HeLa cells, and Nup153 was cleaved to produce a 130-kDa cleavage product that was not observed in cells infected with poliovirus or rhinovirus. The finding that both poliovirus and rhinovirus cause inhibition of nuclear import and degradation of NPC components suggests that this may be a common feature of the replicative cycle of picornaviruses. Inhibition of nuclear import is predicted to result in the cytoplasmic accumulation of a large number of nuclear proteins that could have functions in viral translation, RNA synthesis, packaging, or assembly. Additionally, inhibition of nuclear import also presents a novel strategy whereby cytoplasmic RNA viruses can evade host immune defenses by preventing signal transduction into the nucleus.

Original languageEnglish (US)
Pages (from-to)8787-8796
Number of pages10
JournalJournal of Virology
Volume76
Issue number17
DOIs
StatePublished - 2002
Externally publishedYes

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nucleoporins
Nuclear Pore
Rhinovirus
Enterovirus
Cell Nucleus Active Transport
imports
Enterovirus C
Poliovirus
nuclear proteins
cells
Nuclear Proteins
degradation
nucleocytoplasmic transport
Picornaviridae
RNA Viruses
Viral RNA
Product Packaging
Cytoplasmic and Nuclear Receptors
HeLa Cells
translation (genetics)

ASJC Scopus subject areas

  • Immunology

Cite this

Inhibition of nuclear import and alteration of nuclear pore complex composition by rhinovirus. / Gustin, Kurt E; Sarnow, Peter.

In: Journal of Virology, Vol. 76, No. 17, 2002, p. 8787-8796.

Research output: Contribution to journalArticle

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