Inhibition of premature death by isothiocyanates through immune restoration in LP-BM5 leukemia retrovirus-infected C57BL/6 Mice

Jin Nyoung Ho, Eun Ryung Kang, Ho Geun Yoon, Hyelin Jeon, Woojin Jun, Ronald R Watson, Jeongmin Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The purpose of this study was to determine the effect of isothiocyanates (ITCs) in delaying the progression of the murine immunodeficiency virus to murine AIDS, resulting in increased life span. Furthermore, we investigated the role of ITCs in modulating immune dysfunction caused by LP-BM5 retrovirus infection. Among the tested ITCs, oral administration of sulforaphane (SUL), benzyl isothiocyante (BITC), and phenethyl isothiocyanate (PEITC) showed the inhibition of premature death caused by LP-BM5 retrovirus infection, while indolo[3,2-b] carbazole (ICZ) and indole-3-carbinol (I3C) did not delay the progress ofthe LP-BM5 retrovirus to murine AIDS. Inhibition of premature death by BITC, PEITC, and SUL could be explained by restoration of the immune system and down regulation of free radicals. Dysfunction of T and B cell mitogenesis caused by retrovirus infection in primary cultured splenocytes has been partially recovered with administration of BITC, PEITC, andSUL. There was a shift from imbalanced cytokine production (increased Th2 and decreased Th1 cell cytokine production) into balanced Th1/Th2 cell secretion of cytokines under administration of these ITCs during the development of murine AIDS. Hepatic vitamin E level was significantly restored by administration of these ITCs, in accordance with reduced hepatic lipid peroxidation levels. This study suggests that certain types of ITCs have beneficial effects in preventing premature death during progression to murine AIDS by restoration of immune dysfunction and removal of excessive free radicals, implying that selectiveusage of ITCs would be helpful in retarding the progression from HIV infection to AIDS.

Original languageEnglish (US)
Pages (from-to)1234-1239
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume75
Issue number7
DOIs
StatePublished - 2011

Fingerprint

Isothiocyanates
Premature Mortality
Retroviridae
Inbred C57BL Mouse
Murine Acquired Immunodeficiency Syndrome
Restoration
Leukemia
Retroviridae Infections
Th1 Cells
Cytokines
Free Radicals
Th2 Cells
Immune system
Liver
Vitamin E
Viruses
Lipid Peroxidation
HIV Infections
Oral Administration
Immune System

Keywords

  • Immune dysfunction
  • Isothiocyanates
  • Murine AIDS
  • Oxidative stress
  • Th1 and Th2 cytokines

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Inhibition of premature death by isothiocyanates through immune restoration in LP-BM5 leukemia retrovirus-infected C57BL/6 Mice. / Ho, Jin Nyoung; Kang, Eun Ryung; Yoon, Ho Geun; Jeon, Hyelin; Jun, Woojin; Watson, Ronald R; Lee, Jeongmin.

In: Bioscience, Biotechnology and Biochemistry, Vol. 75, No. 7, 2011, p. 1234-1239.

Research output: Contribution to journalArticle

Ho, Jin Nyoung ; Kang, Eun Ryung ; Yoon, Ho Geun ; Jeon, Hyelin ; Jun, Woojin ; Watson, Ronald R ; Lee, Jeongmin. / Inhibition of premature death by isothiocyanates through immune restoration in LP-BM5 leukemia retrovirus-infected C57BL/6 Mice. In: Bioscience, Biotechnology and Biochemistry. 2011 ; Vol. 75, No. 7. pp. 1234-1239.
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