Inhibition of the Arabidopsis Salt Overly Sensitive Pathway by 14-3-3 proteins

Huapeng Zhou, Huixin Lin, She Chen, Katia Becker, Yongqing Yang, Jinfeng Zhao, Jörg Kudla, Karen S Schumaker, Yan Guo

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

The Salt Overly Sensitive (SOS) pathway regulates intracellular sodium ion (Na+) homeostasis and salt tolerance in plants. Until recently, little was known about the mechanisms that inhibit the SOS pathway when plants are grown in the absence of salt stress. In this study, we report that the Arabidopsis thaliana 14-3-3 proteins λ and κ interact with SOS2 and repress its kinase activity. Growth in the presence of salt decreases the interaction between SOS2 and the 14-3-3 proteins, leading to kinase activation in planta. 14-3-3 λ interacts with the SOS2 junction domain, which is important for its kinase activity. A phosphorylation site (Ser-294) is identified within this domain by mass spectrometry. Mutation of Ser-294 to Ala or Asp does not affect SOS2 kinase activity in the absence of the 14-3-3 proteins. However, in the presence of 14-3-3 proteins, the inhibition of SOS2 activity is decreased by the Ser-to-Ala mutation and enhanced by the Ser-to-Asp exchange. These results identify 14-3-3 λ and κ as important regulators of salt tolerance. The inhibition of SOS2 mediated by the binding of 14-3-3 proteins represents a novel mechanism that confers basal repression of the SOS pathway in the absence of salt stress.

Original languageEnglish (US)
Pages (from-to)1166-1182
Number of pages17
JournalPlant Cell
Volume26
Issue number3
DOIs
StatePublished - 2014

Fingerprint

14-3-3 Proteins
Arabidopsis
Salts
salts
phosphotransferases (kinases)
Phosphotransferases
Salt-Tolerance
salt tolerance
proteins
salt stress
mutation
Mutation
Plantae
homeostasis
phosphorylation
Arabidopsis thaliana
sodium
mass spectrometry
Mass Spectrometry
ions

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Medicine(all)

Cite this

Zhou, H., Lin, H., Chen, S., Becker, K., Yang, Y., Zhao, J., ... Guo, Y. (2014). Inhibition of the Arabidopsis Salt Overly Sensitive Pathway by 14-3-3 proteins. Plant Cell, 26(3), 1166-1182. https://doi.org/10.1105/tpc.113.117069

Inhibition of the Arabidopsis Salt Overly Sensitive Pathway by 14-3-3 proteins. / Zhou, Huapeng; Lin, Huixin; Chen, She; Becker, Katia; Yang, Yongqing; Zhao, Jinfeng; Kudla, Jörg; Schumaker, Karen S; Guo, Yan.

In: Plant Cell, Vol. 26, No. 3, 2014, p. 1166-1182.

Research output: Contribution to journalArticle

Zhou, H, Lin, H, Chen, S, Becker, K, Yang, Y, Zhao, J, Kudla, J, Schumaker, KS & Guo, Y 2014, 'Inhibition of the Arabidopsis Salt Overly Sensitive Pathway by 14-3-3 proteins', Plant Cell, vol. 26, no. 3, pp. 1166-1182. https://doi.org/10.1105/tpc.113.117069
Zhou, Huapeng ; Lin, Huixin ; Chen, She ; Becker, Katia ; Yang, Yongqing ; Zhao, Jinfeng ; Kudla, Jörg ; Schumaker, Karen S ; Guo, Yan. / Inhibition of the Arabidopsis Salt Overly Sensitive Pathway by 14-3-3 proteins. In: Plant Cell. 2014 ; Vol. 26, No. 3. pp. 1166-1182.
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