Inhibitory effect of selenomethionine on the growth of three selected human tumor cell lines

Claire Redman, Julie A. Scott, Antonio T. Baines, Jenny L. Basye, Larry C. Clark, Cindy Calley, Denise Roe, Claire M. Payne, Mark A. Nelson

Research output: Contribution to journalArticle

164 Scopus citations

Abstract

Selenium supplementation has been shown for many years to work as an anticarcinogenic agent both in epidemiology and in in vitro studies. Selenium supplementation has recently been shown to decrease total cancer incidence. However, the mechanism of action of selenium as an anticarcinogenic agent has yet to be elucidated. Selenomethionine was the predominant form of selenium in the dietary supplement in the study by Clark et al. and therefore we evaluated the growth inhibitory effects of selenomethionine against human tumor cells. Selenomethionine was tested against each of three human tumor cell lines (MCF-7/S breast carcinoma, DU-145 prostate cancer cells and UACC-375 melanoma) and against normal human diploid fibroblasts. All cell lines demonstrated a dose-dependent manner of growth inhibition by selenomethionine. Selenomethionine inhibited the growth of all of the human tumor cell lines in the micromolar (μM) range (ranging from 45 to 130 μM) while growth inhibition of normal diploid fibroblasts required 1 mM selenomethionine, approximately 1000-fold higher than for the cancer cell lines. In short, normal diploid fibroblasts were less sensitive than the cancer cell lines to the growth inhibitory effects of selenomethionine. Furthermore, we show that selenomethionine administration to these cancer cell lines results in apoptotic cell death and aberrant mitoses. These results demonstrate the differential sensitivity of tumor cells and normal cells to selenomethionine.

Original languageEnglish (US)
Pages (from-to)103-110
Number of pages8
JournalCancer Letters
Volume125
Issue number1-2
DOIs
StatePublished - Mar 13 1998

Keywords

  • Apoptosis
  • Cancer chemoprevention
  • Growth inhibitory activity
  • Human tumor cell lines
  • Selenomethionine

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Redman, C., Scott, J. A., Baines, A. T., Basye, J. L., Clark, L. C., Calley, C., Roe, D., Payne, C. M., & Nelson, M. A. (1998). Inhibitory effect of selenomethionine on the growth of three selected human tumor cell lines. Cancer Letters, 125(1-2), 103-110. https://doi.org/10.1016/S0304-3835(97)00497-7