Initial appearance of myomesin in differentiating muscle cells

Matthias Ernst, Hans Eppenberger, Thomas C Doetschman

Research output: Contribution to journalArticle

Abstract

The time between the appearance of the striated-muscle-specific M-line protein myomesin and the previous mitosis was measured in individual chick breast muscle cells. The shortest time interval (16 h) was measured with time-lapse cinematography followed by indirect immunofluorescence on 84 cells during the first two days of culture. During these experiments diffuse, cell border and cross-striated fluorescent patterns were observed in both bipolar and non-bipolar cells. A quantitative comparison of the spatial distribution of myomesin to cell morphology and time of culture revealed considerable variation among individual cells. These results indicate that the mechanisms regulating these factors during terminal differentiation are separable and not strictly coordinated.

Original languageEnglish (US)
Pages (from-to)319-323
Number of pages5
JournalCell Differentiation
Volume13
Issue number4
DOIs
StatePublished - 1983
Externally publishedYes

Fingerprint

Connectin
Muscle Cells
Cineradiography
Striated Muscle
Indirect Fluorescent Antibody Technique
Microvilli
Mitosis
Breast

Keywords

  • M-line
  • myogenesis
  • myomesin
  • time-lapse cinematography

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Initial appearance of myomesin in differentiating muscle cells. / Ernst, Matthias; Eppenberger, Hans; Doetschman, Thomas C.

In: Cell Differentiation, Vol. 13, No. 4, 1983, p. 319-323.

Research output: Contribution to journalArticle

Ernst, Matthias ; Eppenberger, Hans ; Doetschman, Thomas C. / Initial appearance of myomesin in differentiating muscle cells. In: Cell Differentiation. 1983 ; Vol. 13, No. 4. pp. 319-323.
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