Initial mapping of the chicken blood plasma proteome

A. Corzo, M. T. Kidd, G. T. Pharr, Shane C Burgess

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Proteomics is the study of the entire protein compliment of an organism. The blood plasma is the only tissue in which an organism's entire proteome may be potentially represented. First results toward mapping the broiler plasma proteome are presented here. Blood was taken from eight 18 day-old representative commercial broiler chickens. Plasma was isolated from each sample and pooled. For initial sample fractioning a 0.4 μl aliquot of the pooled plasma was run on one dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis. Based on relative amounts of protein, the gel was divided into three fractions. The proteins were in-gel digested with trypsin. Two-dimensional liquid chromatography in-line with electrospray ionization tandem mass spectrometry was then used for "shot-gun" qualitative plasma proteomics. The resulting tandem mass spectra were then searched against the non-redundant chicken protein database. Generally accepted high stringency statistical criteria for protein identification were used. Eighty-four chicken proteins were identified. Our work demonstrates the future potential for plasma proteomics for identifying biomarkers of disease and production in chickens.

Original languageEnglish (US)
Pages (from-to)157-162
Number of pages6
JournalInternational Journal of Poultry Science
Volume3
Issue number3
DOIs
StatePublished - 2004
Externally publishedYes

Fingerprint

Proteome
proteome
blood plasma
Chickens
chickens
Proteomics
proteomics
proteins
Proteins
broiler chickens
Gels
gels
Protein Databases
poultry diseases
Electrospray Ionization Mass Spectrometry
poultry production
organisms
Firearms
Tandem Mass Spectrometry
Liquid Chromatography

Keywords

  • Blood plasma
  • Broiler
  • Electrospray ionization
  • Mass spectrometry
  • Proteomics

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology

Cite this

Initial mapping of the chicken blood plasma proteome. / Corzo, A.; Kidd, M. T.; Pharr, G. T.; Burgess, Shane C.

In: International Journal of Poultry Science, Vol. 3, No. 3, 2004, p. 157-162.

Research output: Contribution to journalArticle

Corzo, A. ; Kidd, M. T. ; Pharr, G. T. ; Burgess, Shane C. / Initial mapping of the chicken blood plasma proteome. In: International Journal of Poultry Science. 2004 ; Vol. 3, No. 3. pp. 157-162.
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