Initiation of delayed ovotoxicity by in vitro and in vivo exposures of rat ovaries to 4-vinylcyclohexene diepoxide

Patrick J. Devine, I. Glenn Sipes, Patricia B. Hoyer

Research output: Contribution to journalArticlepeer-review

34 Scopus citations

Abstract

Repeated daily dosing of rats with the ovotoxic, occupational chemical 4-vinylcyclohexene diepoxide (VCD, 80 mg/kg, i.p.) selectively depletes primordial and primary ovarian follicles. This study was designed to investigate whether follicle loss can be achieved following a single, acute exposure to VCD. Ovaries removed from postnatal-day-4 female Fischer 344 rats were cultured in the absence or presence of VCD for 15 days. Continuous in vitro exposure to VCD (15 days) caused concentration-dependent loss of primordial and small primary follicles. A single exposure to VCD in vitro (30 μM, 24 h) also caused significant losses of primordial and primary follicles 14 days later. Additionally, 28-day-old female rats were given a single injection of VCD (40-320 mg/kg, i.p.). A single dose at 320 mg/kg resulted in substantial loss of all follicle stages beginning 6 days later. Overall, these results demonstrate that an acute exposure to high concentrations/doses of VCD is sufficient to cause subsequent delayed loss of follicles.

Original languageEnglish (US)
Pages (from-to)71-77
Number of pages7
JournalReproductive Toxicology
Volume19
Issue number1
DOIs
StatePublished - Nov 1 2004

Keywords

  • 4-Vinylcyclohexene diepoxide
  • Delayed toxicity
  • In vitro
  • Ovarian culture
  • Ovarian follicle
  • Ovarian toxicant
  • Ovotoxicity
  • Single dose

ASJC Scopus subject areas

  • Toxicology

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