Insect olfactory neurons in vitro: Morphological and immunocytochemical characterization of male-specific antennal receptor cells from developing antennae of male manduca sexta

Monika Stengl, John G Hildebrand

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34 Citations (Scopus)

Abstract

Sex-pheromone components released by Manduca sexta females are detected solely by male-specific olfactory receptor neurons that innervate long sensilla trichodea on the male antennae. To facilitate studies of the development and physiology of these receptor cells, we have produced primary in vitro cultures of cells dissociated from pupal male antennae. These cultures comprise several morphological types of cells, 2 of which have been characterized immunocytochemically with a pair of monoclonal antibodies that were shown previously to recognize certain antigens in olfactory receptor neurons at defined stages of development. The good correlation between in vivo and in vitro expression of these antigens suggests that the immunocytochemically recognized cells are olfactory receptor neurons that follow at least partially their normal course of differentiation in vitro.

Original languageEnglish (US)
Pages (from-to)837-847
Number of pages11
JournalJournal of Neuroscience
Volume10
Issue number3
StatePublished - 1990

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Olfactory Receptor Neurons
Manduca
Insects
Neurons
Sensilla
Sex Attractants
Antigens
Cell Physiological Phenomena
Cell Culture Techniques
Monoclonal Antibodies
In Vitro Techniques

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

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