Inside-out red cell membrane vesicles: Preparation and purification

Theodore L. Steck, Ronald S Weinstein, John H. Straus, Donald F H Wallach

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Abstract

Plasma membranes purified from human red cells were converted into small vesicles by diruption in alkaline buffer of low ionic strength. Most of these vesicles were inside-out. The presence of divalent cations prevented this in inversions. The inside-out vesicles were separated from right-side-out vesicles by centrifugation to equilibrium in dextran density gradients.

Original languageEnglish (US)
Pages (from-to)255-257
Number of pages3
JournalScience
Volume168
Issue number3928
Publication statusPublished - 1970
Externally publishedYes

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Cite this

Steck, T. L., Weinstein, R. S., Straus, J. H., & Wallach, D. F. H. (1970). Inside-out red cell membrane vesicles: Preparation and purification. Science, 168(3928), 255-257.