Inside-out red cell membrane vesicles: Preparation and purification

Theodore L. Steck, Ronald S. Weinstein, John H. Straus, Donald F.H. Wallach

Research output: Contribution to journalArticle

294 Scopus citations

Abstract

Plasma membranes purified from human red cells were converted into small vesicles by diruption in alkaline buffer of low ionic strength. Most of these vesicles were inside-out. The presence of divalent cations prevented this in inversions. The inside-out vesicles were separated from right-side-out vesicles by centrifugation to equilibrium in dextran density gradients.

Original languageEnglish (US)
Pages (from-to)255-257
Number of pages3
JournalScience
Volume168
Issue number3928
DOIs
StatePublished - Jan 1 1970
Externally publishedYes

ASJC Scopus subject areas

  • General

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