Inside-out red cell membrane vesicles

Preparation and purification

Theodore L. Steck, Ronald S Weinstein, John H. Straus, Donald F H Wallach

Research output: Contribution to journalArticle

291 Citations (Scopus)

Abstract

Plasma membranes purified from human red cells were converted into small vesicles by diruption in alkaline buffer of low ionic strength. Most of these vesicles were inside-out. The presence of divalent cations prevented this in inversions. The inside-out vesicles were separated from right-side-out vesicles by centrifugation to equilibrium in dextran density gradients.

Original languageEnglish (US)
Pages (from-to)255-257
Number of pages3
JournalScience
Volume168
Issue number3928
StatePublished - 1970
Externally publishedYes

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Divalent Cations
Dextrans
Centrifugation
Osmolar Concentration
Buffers
Cell Membrane

ASJC Scopus subject areas

  • General

Cite this

Steck, T. L., Weinstein, R. S., Straus, J. H., & Wallach, D. F. H. (1970). Inside-out red cell membrane vesicles: Preparation and purification. Science, 168(3928), 255-257.

Inside-out red cell membrane vesicles : Preparation and purification. / Steck, Theodore L.; Weinstein, Ronald S; Straus, John H.; Wallach, Donald F H.

In: Science, Vol. 168, No. 3928, 1970, p. 255-257.

Research output: Contribution to journalArticle

Steck, TL, Weinstein, RS, Straus, JH & Wallach, DFH 1970, 'Inside-out red cell membrane vesicles: Preparation and purification', Science, vol. 168, no. 3928, pp. 255-257.
Steck TL, Weinstein RS, Straus JH, Wallach DFH. Inside-out red cell membrane vesicles: Preparation and purification. Science. 1970;168(3928):255-257.
Steck, Theodore L. ; Weinstein, Ronald S ; Straus, John H. ; Wallach, Donald F H. / Inside-out red cell membrane vesicles : Preparation and purification. In: Science. 1970 ; Vol. 168, No. 3928. pp. 255-257.
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