The long-sought pathway by which selenocysteyl-tRNA[Ser]Sec is synthesized in eukaryotes has been revealed. Seryl-tRNA[Ser]Sec is O-phosphorylated and SecS, a pyridoxal phosphate-dependent protein, catalyzes the reaction of O-phosphoseryl-tRNA[Ser]Sec with monoselenophosphate to give selenocysteyl-tRNA[Ser]Sec. 1H-77Se HMQC-TOCSY NMR spectroscopy has been developed to detect the selenium-containing amino acids present in selenized yeast after protease XIV digestion. An archived selenized yeast sample is found to contain the novel amino acid S-(methylseleno)cysteine in addition to selenomethionine. Arsenite and selenite react with GSH to form (GS)2AsSe-. The structure of this compound has been determined by EXAFS, 77Se NMR and Raman spectroscopic and chromatographic studies. Its formation under biological conditions has been demonstrated.
|Original language||English (US)|
|Number of pages||7|
|Journal||Phosphorus, Sulfur and Silicon and the Related Elements|
|State||Published - Apr 2008|
ASJC Scopus subject areas
- Organic Chemistry
- Inorganic Chemistry