Instant scanner device for identifying wound infection utilizing Mie scatter spectra

Robin E. Sweeney, Elizabeth Budiman, Jeong Yeol Yoon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Tissue biopsy and swab culture are the gold standards for diagnosing tissue infection; these tests require significant time, diagnostic costs, and resources. Towards earlier and specific diagnosis of infection, a non-destructive, rapid, and mobile detection device is described to distinguish bacterial species via light scatter spectra from the surface of an infected tissue, reagent-free. Porcine skin and human cadaveric skin models of wound infection were used with a 650 nm LED and an angular photodiode array to detect bacterial infections on the tissue surface, which can easily be translated to a typical CMOS array or smartphone. Tissue samples were inoculated with Escherichia coli, Salmonella Typhimurium, or Staphylococcus aureus and backscatter was collected from 100° to 170° in 10° increments; each bacterial species resulted in unique Mie scatter spectra. Distinct Mie scatter spectra were obtained from epidermis (intact skin model) and dermis (wound model) samples, as well as from porcine and human cadaveric skin samples. Interactions between bacterial colonies and lipid particles within dermis samples generated a characteristic Mie scatter spectrum, while the lipid itself did not contribute to such characteristic spectrum as corroborated with body lotion experiments. The designed angular photodiode array is able to immediately and non-destructively detect tissue bacterial infection and identify the species of infection within three seconds, which could greatly improve point of care diagnostics and antibiotic treatments.

Original languageEnglish (US)
Title of host publicationAdvanced Environmental, Chemical, and Biological Sensing Technologies XIV
EditorsTuan Vo-Dinh, Robert A. Lieberman
PublisherSPIE
ISBN (Electronic)9781510609310
DOIs
StatePublished - 2017
EventAdvanced Environmental, Chemical, and Biological Sensing Technologies XIV 2017 - Anaheim, United States
Duration: Apr 9 2017Apr 10 2017

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume10215
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Other

OtherAdvanced Environmental, Chemical, and Biological Sensing Technologies XIV 2017
CountryUnited States
CityAnaheim
Period4/9/174/10/17

Keywords

  • Diabetic foot ulcer
  • Escherichia coli
  • Light scatter
  • Salmonella Typhimurium
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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