Insulin attenuates vasopressin-induced calcium transients and a voltage-dependent calcium response in rat vascular smooth muscle cells

P. R. Standley, F. Zhang, J. L. Ram, M. B. Zemel, J. R. Sowers

Research output: Contribution to journalArticle

123 Scopus citations

Abstract

Insulin attenuates the contractile responses of vascular smooth muscle (VSM) to various agonists. Insulinopenic and insulin-resistant rats lack this normal attenuation of vascular contractile responses. To study this attenuating mechanism, the effects of insulin on calcium (Ca2+) responses of cultured VSM cells (a7r5) to arginine vasopressin (AVP) and membrane potential were investigated. Insulin (1 and 100 mU/ml) shifted AVP dose-response curves to the right, reducing relative potency of AVP by 16-fold and 220-fold, respectively. Responses to AVP were significantly attenuated within 30 min of insulin application. The AVP-elicited rise in [Ca2+](i) was partially dependent upon extracellular Ca2+. AVP-elicited inward current was reduced by 90 min of insulin treatment (100 mU/ml), from a peak current of -103±27 pA (normal) to -37±15 pA (insulin treated). Peak voltage-dependent Ca2+-dependent inward current was unaffected by insulin; however, the current-voltage curve was shifted 16±3 mV to the right by insulin. Thus, insulin may reduce VSM contractile responses by attenuating agonist-mediated rises in [Ca2+](i) mediated, in part, by reductions in Ca2+ influx through both receptor- and voltage-operated channels.

Original languageEnglish (US)
Pages (from-to)1230-1236
Number of pages7
JournalJournal of Clinical Investigation
Volume88
Issue number4
DOIs
StatePublished - 1991

Keywords

  • Diabetes
  • Fura-2
  • Hypertension
  • Whole cell recording

ASJC Scopus subject areas

  • Medicine(all)

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