Insulin-like peptides in the mosquito Anopheles stephensi: Identification and expression in response to diet and infection with Plasmodium falciparum

Alexander G. Marquez, Jose E. Pietri, Hannah M. Smithers, Andrew Nuss, Yevgeniya Antonova, Anna L. Drexler, Michael A Riehle, Mark R. Brown, Shirley Luckhart

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Insulin-like peptides (ILPs) regulate a multitude of biological processes, including metabolism and immunity to infection, and share similar structural motifs across widely divergent taxa. Insulin/insulin-like growth factor signaling (IIS) pathway elements are similarly conserved. We have shown that IIS regulates reproduction, innate immunity, and lifespan in female Anopheles stephensi, a major mosquito vector of human malaria. To further explore IIS regulation of these processes, we identified genes encoding five ILPs in this species and characterized their expression in tissues. Antisera to ILP homologs in Anopheles gambiae were used to identify cellular sources in An. stephensi females by immunocytochemistry. We analyzed tissue-specific ILP transcript expression in young and older females, in response to different feeding regimens, and in response to infection with Plasmodium falciparum with quantitative reverse transcriptase-PCR assays. While some ILP transcript changes were evident in older females and in response to blood feeding, significant changes were particularly notable in response to hormonal concentrations of ingested human insulin and to P. falciparum infection. These changes suggest that ILP secretion and action may be similarly responsive in Plasmodium-infected females and potentially alter metabolism and innate immunity.

Original languageEnglish (US)
Pages (from-to)303-312
Number of pages10
JournalGeneral and Comparative Endocrinology
Volume173
Issue number2
DOIs
StatePublished - Sep 1 2011

Fingerprint

Anopheles stephensi
Anopheles
Plasmodium falciparum
Culicidae
insulin
peptides
Insulin
Diet
Peptides
Infection
diet
infection
Innate Immunity
Malaria
Anopheles gambiae
Biological Phenomena
metabolism
Plasmodium
somatomedins
immunocytochemistry

Keywords

  • Anopheles
  • Insect
  • Insulin signaling
  • Malaria
  • Mosquito
  • Plasmodium

ASJC Scopus subject areas

  • Endocrinology

Cite this

Insulin-like peptides in the mosquito Anopheles stephensi : Identification and expression in response to diet and infection with Plasmodium falciparum. / Marquez, Alexander G.; Pietri, Jose E.; Smithers, Hannah M.; Nuss, Andrew; Antonova, Yevgeniya; Drexler, Anna L.; Riehle, Michael A; Brown, Mark R.; Luckhart, Shirley.

In: General and Comparative Endocrinology, Vol. 173, No. 2, 01.09.2011, p. 303-312.

Research output: Contribution to journalArticle

Marquez, Alexander G. ; Pietri, Jose E. ; Smithers, Hannah M. ; Nuss, Andrew ; Antonova, Yevgeniya ; Drexler, Anna L. ; Riehle, Michael A ; Brown, Mark R. ; Luckhart, Shirley. / Insulin-like peptides in the mosquito Anopheles stephensi : Identification and expression in response to diet and infection with Plasmodium falciparum. In: General and Comparative Endocrinology. 2011 ; Vol. 173, No. 2. pp. 303-312.
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