Intact biliary excretion of gastrically administered prostaglandin F(2α) in rats: Developmental differences

Alan D Bedrick, M. A. Wells, D. L. Ford, O. Koldovsky

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Abstract

Tritium-labeled prostaglandin F(2α) was administered via orogastric tube to bile duct-cannulated suckling and weanling rats to determine if maturational differences were present in the biliary excretion of prostaglandin F(2α) and metabolites. Animals were killed 2 h after radioactivity administration. Characterization of radioactivity present in bile revealed age-related differences in biliary prostaglandin F(2α) excretion. Suckling rats had a greater proportion of radioactivity migrating in chromatographic regions of greater polarity than prostaglandin F(2α). Compared with the weanling, a significantly greater amount of radioactivity cochromatographed with intact, unmetabolized prostaglandin F(2α) (33.08 ± 1.99 vs. 21.38 ± 1.46). These results indicate that orogastrically administered prostaglandin F(2α) can be absorbed from the gastrointestinal tract, transported to the liver, and subsequently excreted into bile and detected in an unmetabolized form in suckling and weanling rats. The enterohepatic circulation of milk-derived prostaglandin present in bile may contribute to the overall content of intestinal prostaglandins.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Gastrointestinal and Liver Physiology
Volume253
Issue number6
StatePublished - 1987

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Prostaglandins F
Radioactivity
Bile
Prostaglandins
Enterohepatic Circulation
Gastrointestinal Contents
Tritium
Bile Ducts
Gastrointestinal Tract
Hepatobiliary Elimination
Milk
Liver

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology

Cite this

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title = "Intact biliary excretion of gastrically administered prostaglandin F(2α) in rats: Developmental differences",
abstract = "Tritium-labeled prostaglandin F(2α) was administered via orogastric tube to bile duct-cannulated suckling and weanling rats to determine if maturational differences were present in the biliary excretion of prostaglandin F(2α) and metabolites. Animals were killed 2 h after radioactivity administration. Characterization of radioactivity present in bile revealed age-related differences in biliary prostaglandin F(2α) excretion. Suckling rats had a greater proportion of radioactivity migrating in chromatographic regions of greater polarity than prostaglandin F(2α). Compared with the weanling, a significantly greater amount of radioactivity cochromatographed with intact, unmetabolized prostaglandin F(2α) (33.08 ± 1.99 vs. 21.38 ± 1.46). These results indicate that orogastrically administered prostaglandin F(2α) can be absorbed from the gastrointestinal tract, transported to the liver, and subsequently excreted into bile and detected in an unmetabolized form in suckling and weanling rats. The enterohepatic circulation of milk-derived prostaglandin present in bile may contribute to the overall content of intestinal prostaglandins.",
author = "Bedrick, {Alan D} and Wells, {M. A.} and Ford, {D. L.} and O. Koldovsky",
year = "1987",
language = "English (US)",
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journal = "American Journal of Physiology",
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T1 - Intact biliary excretion of gastrically administered prostaglandin F(2α) in rats

T2 - Developmental differences

AU - Bedrick, Alan D

AU - Wells, M. A.

AU - Ford, D. L.

AU - Koldovsky, O.

PY - 1987

Y1 - 1987

N2 - Tritium-labeled prostaglandin F(2α) was administered via orogastric tube to bile duct-cannulated suckling and weanling rats to determine if maturational differences were present in the biliary excretion of prostaglandin F(2α) and metabolites. Animals were killed 2 h after radioactivity administration. Characterization of radioactivity present in bile revealed age-related differences in biliary prostaglandin F(2α) excretion. Suckling rats had a greater proportion of radioactivity migrating in chromatographic regions of greater polarity than prostaglandin F(2α). Compared with the weanling, a significantly greater amount of radioactivity cochromatographed with intact, unmetabolized prostaglandin F(2α) (33.08 ± 1.99 vs. 21.38 ± 1.46). These results indicate that orogastrically administered prostaglandin F(2α) can be absorbed from the gastrointestinal tract, transported to the liver, and subsequently excreted into bile and detected in an unmetabolized form in suckling and weanling rats. The enterohepatic circulation of milk-derived prostaglandin present in bile may contribute to the overall content of intestinal prostaglandins.

AB - Tritium-labeled prostaglandin F(2α) was administered via orogastric tube to bile duct-cannulated suckling and weanling rats to determine if maturational differences were present in the biliary excretion of prostaglandin F(2α) and metabolites. Animals were killed 2 h after radioactivity administration. Characterization of radioactivity present in bile revealed age-related differences in biliary prostaglandin F(2α) excretion. Suckling rats had a greater proportion of radioactivity migrating in chromatographic regions of greater polarity than prostaglandin F(2α). Compared with the weanling, a significantly greater amount of radioactivity cochromatographed with intact, unmetabolized prostaglandin F(2α) (33.08 ± 1.99 vs. 21.38 ± 1.46). These results indicate that orogastrically administered prostaglandin F(2α) can be absorbed from the gastrointestinal tract, transported to the liver, and subsequently excreted into bile and detected in an unmetabolized form in suckling and weanling rats. The enterohepatic circulation of milk-derived prostaglandin present in bile may contribute to the overall content of intestinal prostaglandins.

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