Integrated microfluidic system for rapid DNA fingerprint analysis: A miniaturized integrated DNA analysis system (MiDAS)—Swab sample-in to DNA profile-out

Jianing Yang, Cedric Hurth, Alan Nordquist, Stan Smith, Frederic Zenhausern

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A fully automated rapid DNA analysis system requires integrating several operational elements performing multiple steps onto one single microfluidic platform. The functions to include on the microfluidic platform consist of substrate lysis, lysate DNA extraction, single or multiplexed PCR amplification, amplicon separation, and product readout. Here we describe a fully automated integrated system for forensic short tandem repeat (STR) analysis of reference samples, achieving buccal swab-in and DNA profile-out.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages207-224
Number of pages18
DOIs
Publication statusPublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1906
ISSN (Print)1064-3745

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Keywords

  • Capillary electrophoresis
  • DNA extraction
  • Integration
  • Microfluidics
  • Multiplexed PCR
  • STR analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Yang, J., Hurth, C., Nordquist, A., Smith, S., & Zenhausern, F. (2019). Integrated microfluidic system for rapid DNA fingerprint analysis: A miniaturized integrated DNA analysis system (MiDAS)—Swab sample-in to DNA profile-out. In Methods in Molecular Biology (pp. 207-224). (Methods in Molecular Biology; Vol. 1906). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-8964-5_14