Integrative transcriptomic and genomic analysis of odorant binding proteins and chemosensory proteins in aphids

Q. Wang, J. J. Zhou, J. T. Liu, G. Z. Huang, W. Y. Xu, Q. Zhang, J. L. Chen, Y. J. Zhang, Xianchun Li, S. H. Gu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Odorant binding proteins (OBPs) and chemosensory proteins (CSPs) play essential roles in insect chemosensory recognition. Here, we identified nine OBPs and nine CSPs from the Myzus persicae transcriptome and genome. Genomic structure analysis showed that the number and length of the introns are much higher, and this appears to be a unique feature of aphid OBP genes. Three M. persicae OBP genes (OBP3/7/8) as well as CSP1/4/6, CSP2/9 and CSP5/8 are tandem arrayed in the genome. Phylogenetic analyses of five different aphid species suggest that aphid OBPs and CSPs are conserved in single copy across all aphids (with occasional losses), indicating that each OBP and CSP class evolved from a single gene in the common ancestor of aphids without subsequent duplication. Motif pattern analysis revealed that aphid OBP and CSP motifs are highly conserved, and this could suggest the conserved functions of aphid OBPs and CSPs. Three OBPs (MperOBP6/7/10) are expressed antennae specifically, and five OBPs (MperOBP2/4/5/8/9) are expressed antennae enriched, consistent with their putative olfactory roles. M. persicae CSPs showed much broader expression profiles in nonsensory organs than OBPs. None of the nine MperCSPs were found to be antennae specific, but five of them (MperCSP1/2/4/5/6) showed higher expression levels in the legs than in other tissues. MperCSP10 mainly expressed in the antennae and legs. The broad and diverse expression patterns of M. persicae CSPs suggest their multifunctions in olfactory perception, development and other processes.

Original languageEnglish (US)
JournalInsect Molecular Biology
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Aphids
transcriptomics
Aphidoidea
genomics
Proteins
proteins
antennae
Leg
legs
odorant receptors
odorant-binding protein
Olfactory Perception
Genome
Genes
Amino Acid Motifs
genome
genes
Myzus persicae
Transcriptome
transcriptome

Keywords

  • chemosensory protein
  • genomic structure
  • motif-pattern
  • Myzus persicae
  • odorant binding protein
  • tissue expression profiles

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Insect Science

Cite this

Integrative transcriptomic and genomic analysis of odorant binding proteins and chemosensory proteins in aphids. / Wang, Q.; Zhou, J. J.; Liu, J. T.; Huang, G. Z.; Xu, W. Y.; Zhang, Q.; Chen, J. L.; Zhang, Y. J.; Li, Xianchun; Gu, S. H.

In: Insect Molecular Biology, 01.01.2018.

Research output: Contribution to journalArticle

Wang, Q. ; Zhou, J. J. ; Liu, J. T. ; Huang, G. Z. ; Xu, W. Y. ; Zhang, Q. ; Chen, J. L. ; Zhang, Y. J. ; Li, Xianchun ; Gu, S. H. / Integrative transcriptomic and genomic analysis of odorant binding proteins and chemosensory proteins in aphids. In: Insect Molecular Biology. 2018.
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abstract = "Odorant binding proteins (OBPs) and chemosensory proteins (CSPs) play essential roles in insect chemosensory recognition. Here, we identified nine OBPs and nine CSPs from the Myzus persicae transcriptome and genome. Genomic structure analysis showed that the number and length of the introns are much higher, and this appears to be a unique feature of aphid OBP genes. Three M. persicae OBP genes (OBP3/7/8) as well as CSP1/4/6, CSP2/9 and CSP5/8 are tandem arrayed in the genome. Phylogenetic analyses of five different aphid species suggest that aphid OBPs and CSPs are conserved in single copy across all aphids (with occasional losses), indicating that each OBP and CSP class evolved from a single gene in the common ancestor of aphids without subsequent duplication. Motif pattern analysis revealed that aphid OBP and CSP motifs are highly conserved, and this could suggest the conserved functions of aphid OBPs and CSPs. Three OBPs (MperOBP6/7/10) are expressed antennae specifically, and five OBPs (MperOBP2/4/5/8/9) are expressed antennae enriched, consistent with their putative olfactory roles. M. persicae CSPs showed much broader expression profiles in nonsensory organs than OBPs. None of the nine MperCSPs were found to be antennae specific, but five of them (MperCSP1/2/4/5/6) showed higher expression levels in the legs than in other tissues. MperCSP10 mainly expressed in the antennae and legs. The broad and diverse expression patterns of M. persicae CSPs suggest their multifunctions in olfactory perception, development and other processes.",
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AU - Zhang, Q.

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KW - genomic structure

KW - motif-pattern

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KW - tissue expression profiles

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