Integrin α6β4E variant is associated with actin and CD9 structures and modifies the biophysical properties of cell-cell and cell-extracellular matrix interactions

Mengdie Wang, James P. Hinton, Jaime M.C. Gard, Joe GN Garcia, Beatrice S. Knudsen, Raymond B Nagle, Anne E Cress

Research output: Contribution to journalArticle

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Abstract

Integrin α6β4 is an essential, dynamic adhesion receptor for laminin 332 found on epithelial cells, required for formation of strong cell-extracellular matrix (ECM) adhesion and induced migration, and coordinated by regions of the β4C cytoplasmic domain. β4E, a unique splice variant of β4 expressed in normal tissue, contains a cytoplasmic domain of 231 amino acids with a unique sequence of 114 amino acids instead of β4C's canonical 1089 amino acids. We determined the distribution of α6β4E within normal human glandular epithelium and its regulation and effect on cellular biophysical properties. Canonical α6β4C expressed in all basal cells, as expected, while α6β4E expressed within a subset of luminal cells. α6β4E expression was induced by three-dimensional culture conditions, activated Src, was reversible, and was stabilized by bortezomib, a proteasome inhibitor. α6β4C expressed in all cells during induced migration, whereas α6β4E was restricted to a subset of cells with increased kinetics of cell-cell and cell-ECM resistance properties. Interestingly, α6β4E presented in "ringlike" patterns measuring ∼1.75 × 0.72 microns and containing actin and CD9 at cell-ECM locations. In contrast, α6β4C expressed only within hemidesmosome-like structures containing BP180. Integrin α6β4E is an inducible adhesion isoform in normal epithelial cells that can alter biophysical properties of cell-cell and cell-ECM interactions.

Original languageEnglish (US)
Pages (from-to)838-850
Number of pages13
JournalMolecular biology of the cell
Volume30
Issue number7
DOIs
StatePublished - Mar 21 2019

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Integrins
Extracellular Matrix
Actins
Epithelial Cells
Hemidesmosomes
Cell-Matrix Junctions
Amino Acids
Proteasome Inhibitors
Amino Acid Sequence
Protein Isoforms
Epithelium

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Integrin α6β4E variant is associated with actin and CD9 structures and modifies the biophysical properties of cell-cell and cell-extracellular matrix interactions. / Wang, Mengdie; Hinton, James P.; Gard, Jaime M.C.; Garcia, Joe GN; Knudsen, Beatrice S.; Nagle, Raymond B; Cress, Anne E.

In: Molecular biology of the cell, Vol. 30, No. 7, 21.03.2019, p. 838-850.

Research output: Contribution to journalArticle

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