Integrin α6 expression in human prostate carcinoma cells is associated with a migratory and invasive phenotype in vitro and in vivo

Isaac Rabinovitz, Raymond B Nagle, Anne E Cress

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

Cell adhesion and migration are important features in tumor invasion, being mediated in part by integrins (extracellular matrix receptors). Integrins are significantly decreased in human prostate cancer. An exception is α6 integrin (laminin receptor) which persists during prostate tumor progression. We have selected high (DU-H) and low (DU-L) expressors of α6 integrin from a human prostate tumor cell line, DU145, to assess experimentally the importance of α6 integrin in tumor invasion. DU-H cells exhibited a four-fold increased expression of α6 integrin on the surface compared to DU-L cells. Both cell types contained similar amounts of α3 and α5 integrin. The DU-H cells contained α6 subunits complexed with both the β1 and β4 subunits whereas DU-L cells contained α6 complexed only with β4. DU-H cells were three times more mobile on laminin as compared to DU-L, but adhered similarly on laminin. Adhesion and migration were inhibited with anti-α6 antibody. Each subline was injected intraperitoneally into SCID mice to test its invasive potential. Results showed greater invasion of DU-H compared to DU-L cells, with increased expression of a6 integrin on the tumor at the areas of invasion. These data suggest that α6 integrin expression is advantageous for prostate tumor cell invasion.

Original languageEnglish (US)
Pages (from-to)481-491
Number of pages11
JournalClinical and Experimental Metastasis
Volume13
Issue number6
DOIs
StatePublished - Nov 1995

Fingerprint

Integrins
Prostate
Carcinoma
Phenotype
Laminin
Neoplasms
Laminin Receptors
In Vitro Techniques
SCID Mice
Tumor Cell Line
Cell Adhesion
Cell Movement
Anti-Idiotypic Antibodies
Prostatic Neoplasms

Keywords

  • basement membrane
  • cell adhesion
  • extracellular matrix
  • laminin
  • prostate cancer

ASJC Scopus subject areas

  • Cancer Research

Cite this

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title = "Integrin α6 expression in human prostate carcinoma cells is associated with a migratory and invasive phenotype in vitro and in vivo",
abstract = "Cell adhesion and migration are important features in tumor invasion, being mediated in part by integrins (extracellular matrix receptors). Integrins are significantly decreased in human prostate cancer. An exception is α6 integrin (laminin receptor) which persists during prostate tumor progression. We have selected high (DU-H) and low (DU-L) expressors of α6 integrin from a human prostate tumor cell line, DU145, to assess experimentally the importance of α6 integrin in tumor invasion. DU-H cells exhibited a four-fold increased expression of α6 integrin on the surface compared to DU-L cells. Both cell types contained similar amounts of α3 and α5 integrin. The DU-H cells contained α6 subunits complexed with both the β1 and β4 subunits whereas DU-L cells contained α6 complexed only with β4. DU-H cells were three times more mobile on laminin as compared to DU-L, but adhered similarly on laminin. Adhesion and migration were inhibited with anti-α6 antibody. Each subline was injected intraperitoneally into SCID mice to test its invasive potential. Results showed greater invasion of DU-H compared to DU-L cells, with increased expression of a6 integrin on the tumor at the areas of invasion. These data suggest that α6 integrin expression is advantageous for prostate tumor cell invasion.",
keywords = "basement membrane, cell adhesion, extracellular matrix, laminin, prostate cancer",
author = "Isaac Rabinovitz and Nagle, {Raymond B} and Cress, {Anne E}",
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AU - Nagle, Raymond B

AU - Cress, Anne E

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AB - Cell adhesion and migration are important features in tumor invasion, being mediated in part by integrins (extracellular matrix receptors). Integrins are significantly decreased in human prostate cancer. An exception is α6 integrin (laminin receptor) which persists during prostate tumor progression. We have selected high (DU-H) and low (DU-L) expressors of α6 integrin from a human prostate tumor cell line, DU145, to assess experimentally the importance of α6 integrin in tumor invasion. DU-H cells exhibited a four-fold increased expression of α6 integrin on the surface compared to DU-L cells. Both cell types contained similar amounts of α3 and α5 integrin. The DU-H cells contained α6 subunits complexed with both the β1 and β4 subunits whereas DU-L cells contained α6 complexed only with β4. DU-H cells were three times more mobile on laminin as compared to DU-L, but adhered similarly on laminin. Adhesion and migration were inhibited with anti-α6 antibody. Each subline was injected intraperitoneally into SCID mice to test its invasive potential. Results showed greater invasion of DU-H compared to DU-L cells, with increased expression of a6 integrin on the tumor at the areas of invasion. These data suggest that α6 integrin expression is advantageous for prostate tumor cell invasion.

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