Interaction of ovine somatomedin and multiplication stimulating activity/rat insulin-like growth factor II with cultured skeletal muscle satellite cells

M. V. Dodson, Ronald E Allen, N. Shimizu, Y. Shimizu, K. L. Hossner

Research output: Contribution to journalArticle

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Abstract

The interactions of 125I-multiplication stimulating activity (MSA) and 125I-ovine somatomedin with receptors on skeletal muscle satellite cells are described. Specific binding of 125I-MSA/rIGF-II was inhibited by MSA/rIGF-II and oSm but not by insulin. Binding of 125I-oSm was inhibited by MSA/rIGF-II, oSm and insulin. In addition, 24-h pre-incubation of satellite cells with insulin increased the amount of 125I-MSA/rIGF-II bound, but insulin concentrations below 550 μg/l had no effect on the subsequent binding of 125I-oSm. Pre-incubation of cultures with oSm or MSA/rIGF-II decreased the subsequent binding of 125I-oSm and 125I-MSA/rIGF-II. These preliminary experiments suggest that oSm is similar to IGF-I in its binding characteristics and that primary cultures of skeletal muscle satellite cells possess type I and type II IGF receptors.

Original languageEnglish (US)
Pages (from-to)425-432
Number of pages8
JournalActa Endocrinologica
Volume116
Issue number3
StatePublished - 1987

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Skeletal Muscle Satellite Cells
Insulin-Like Growth Factor II
Somatomedins
Sheep
Insulin
IGF Type 2 Receptor
Somatomedin Receptors
IGF Type 1 Receptor
rat insulin-like growth factor II
Insulin-Like Growth Factor I

ASJC Scopus subject areas

  • Endocrinology

Cite this

Interaction of ovine somatomedin and multiplication stimulating activity/rat insulin-like growth factor II with cultured skeletal muscle satellite cells. / Dodson, M. V.; Allen, Ronald E; Shimizu, N.; Shimizu, Y.; Hossner, K. L.

In: Acta Endocrinologica, Vol. 116, No. 3, 1987, p. 425-432.

Research output: Contribution to journalArticle

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AU - Hossner, K. L.

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AB - The interactions of 125I-multiplication stimulating activity (MSA) and 125I-ovine somatomedin with receptors on skeletal muscle satellite cells are described. Specific binding of 125I-MSA/rIGF-II was inhibited by MSA/rIGF-II and oSm but not by insulin. Binding of 125I-oSm was inhibited by MSA/rIGF-II, oSm and insulin. In addition, 24-h pre-incubation of satellite cells with insulin increased the amount of 125I-MSA/rIGF-II bound, but insulin concentrations below 550 μg/l had no effect on the subsequent binding of 125I-oSm. Pre-incubation of cultures with oSm or MSA/rIGF-II decreased the subsequent binding of 125I-oSm and 125I-MSA/rIGF-II. These preliminary experiments suggest that oSm is similar to IGF-I in its binding characteristics and that primary cultures of skeletal muscle satellite cells possess type I and type II IGF receptors.

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