Interaction of ovine somatomedin and multiplication stimulating activity/rat insulin-like growth factor II with cultured skeletal muscle satellite cells

M. V. Dodson, R. E. Allen, N. Shimizu, Y. Shimizu, K. L. Hossner

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The interactions of 125I-multiplication stimulating activity (MSA) and 125I-ovine somatomedin with receptors on skeletal muscle satellite cells are described. Specific binding of 125I-MSA/rIGF-II was inhibited by MSA/rIGF-II and oSm but not by insulin. Binding of 125I-oSm was inhibited by MSA/rIGF-II, oSm and insulin. In addition, 24-h pre-incubation of satellite cells with insulin increased the amount of 125I-MSA/rIGF-II bound, but insulin concentrations below 550 μg/l had no effect on the subsequent binding of 125I-oSm. Pre-incubation of cultures with oSm or MSA/rIGF-II decreased the subsequent binding of 125I-oSm and 125I-MSA/rIGF-II. These preliminary experiments suggest that oSm is similar to IGF-I in its binding characteristics and that primary cultures of skeletal muscle satellite cells possess type I and type II IGF receptors.

Original languageEnglish (US)
Pages (from-to)425-432
Number of pages8
JournalActa Endocrinologica
Issue number3
StatePublished - Jan 1 1987


ASJC Scopus subject areas

  • Endocrinology

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