Interaction of the adenovirus L1 52/55-kilodalton protein with the IVa2 gene product during infection

Kurt E Gustin, Pierre Lutz, Michael J. Imperiale

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

The adenovirus L1 52/55-kDa protein is expressed both in the early and late stages of infection, raising the possibility that it has multiple roles in the viral life cycle. To obtain possible insights into these roles, the yeast two-hybrid system was used to examine the interactions of the 52/55- kDa protein with viral and cellular factors. cDNA expression libraries from human 293 cells at both early and late stages of adenovirus type 5 infection were constructed and screened, with the 52/55-kDa protein being used as bait. Characterization of positive clones revealed that the adenovirus IVa2 gene product interacted specifically with the 52/55-kDa protein. In addition, the IVa2 protein was shown to interact with a bacterial glutathione S-transferase-52/55-kDa fusion protein in vitro, further supporting the finding with the yeast two-hybrid system. Finally, coimmunoprecipitation studies confirmed that the 52/55-kDa protein and IVa2 polypeptide interact specifically during the course of adenovirus infection. A potential role for the IVa2-52/55-kDa protein interaction in the regulation of transcription from the major late promoter and in viral assembly is discussed.

Original languageEnglish (US)
Pages (from-to)6463-6467
Number of pages5
JournalJournal of Virology
Volume70
Issue number9
StatePublished - Sep 1996
Externally publishedYes

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Adenoviridae
Infection
infection
Genes
Proteins
genes
proteins
Adenoviridae Infections
Two-Hybrid System Techniques
two hybrid system techniques
Virus Assembly
yeasts
Viral Proteins
Glutathione Transferase
Life Cycle Stages
Gene Library
glutathione transferase
baits
Clone Cells
life cycle (organisms)

ASJC Scopus subject areas

  • Immunology

Cite this

Interaction of the adenovirus L1 52/55-kilodalton protein with the IVa2 gene product during infection. / Gustin, Kurt E; Lutz, Pierre; Imperiale, Michael J.

In: Journal of Virology, Vol. 70, No. 9, 09.1996, p. 6463-6467.

Research output: Contribution to journalArticle

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