Interactive toxicity and stress protein expression by vinylidene chloride and monochloroacetate in precision-cut rat liver slices

J. Wijeweera, A Jay Gandolfi, X. H. Zheng

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Vinylidene chloride (VDC) is a groundwater and drinking water contaminant. Monochloroacetic acid (MCA) is a chlorination by-product of drinking water. Because environmental or occupational exposure to chemicals takes place at low concentrations, a sensitive in vitro system of liver slices was used to examine the interactive toxicity of MCA and VDC. Liver slices from Sprague-Dawley rats were exposed to 100 μM MCA for 1 hr before exposure to 20 or 48 μM VDC and incubated for 1 to 8 hr. MCA + 48 μM VDC resulted in a significant leakage of K+ by 4 hr, while MCA + 20 μM VDC did not. At 4 hr, MCA + 48 μM VDC resulted in centrilobular necrosis. MCA caused a significant depletion of slice glutathione (GSH) at 1 hr, which was maintained up to 3 hr. As reactive VDC metabolites are detoxified by conjugation with GSH, the increase in VDC toxicity by MCA is possibly due to GSH-depleting effects of MCA. Heat shock protein (HSP) 72 was increased 2.5-fold by MCA + 20 μM VDC as early as 2 hr, although K+ leakage was not increased. MCA + 48 μM VDC resulted in a 3-fold increase in HSP 72 by 2 hr, while there were modest increases in HSPs 60 and 32. Therefore, HSP 72 is an early sensitive indicator of interactive toxicity of nontoxic concentrations of MCA and VDC. This is the first time that micromolar concentrations of these drinking water contaminants were observed to affect cellular homeostasis in the liver.

Original languageEnglish (US)
Pages (from-to)1319-1323
Number of pages5
JournalEnvironmental Health Perspectives
Volume106
Issue numberSUPPL. 6
StatePublished - 1998

Fingerprint

Heat-Shock Proteins
Liver
Toxicity
Rats
chloride
toxicity
protein
acid
HSP72 Heat-Shock Proteins
heat shock
Drinking Water
drinking water
leakage
vinylidene chloride
chloroacetic acid
Impurities
fold
pollutant
Chlorination
Halogenation

Keywords

  • Drinking water contaminants
  • Glutathione
  • Interactive hepatotoxicity
  • Monochloroacetic acid
  • Stress proteins
  • Vinylidene chloride

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health

Cite this

Interactive toxicity and stress protein expression by vinylidene chloride and monochloroacetate in precision-cut rat liver slices. / Wijeweera, J.; Gandolfi, A Jay; Zheng, X. H.

In: Environmental Health Perspectives, Vol. 106, No. SUPPL. 6, 1998, p. 1319-1323.

Research output: Contribution to journalArticle

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