Intercellular propagation of calcium waves mediated by inositol trisphosphate

Scott A Boitano, Ellen R. Dirksen, Michael J. Sanderson

Research output: Contribution to journalArticle

421 Citations (Scopus)

Abstract

Two types of calcium (Ca2+) signaling - propagating intercellular Ca2+ waves of increasing intracellular Ca2+ concentration ([Ca2+]i) and nonpropagating oscillations in [Ca2+]i - co-exist in a variety of cell types. To investigate this difference in Ca2+ signaling, airway epithelial cells were loaded with heparin, an inositol 1,4,5-trisphosphate (IP3) receptor antagonist, by pulsed, high-frequency electroporation. Heparin inhibited propagation of intercellular Ca2+ waves but not oscillations of [Ca2+]i. In heparin-free cells, Ca2+ waves propagated through cells displaying [Ca2+]i oscillations. Depletion of intracellular Ca2+ pools with the Ca2+-pump inhibitor thapsigargin also inhibited the propagation of Ca2+ waves. These studies demonstrate that the release of Ca2+ by IP3 is necessary for the propagation of intercellular Ca2+ waves and suggest that IP3 moves through gap junctions to communicate intercellular Ca2+ waves.

Original languageEnglish (US)
Pages (from-to)292-295
Number of pages4
JournalScience
Volume258
Issue number5080
StatePublished - Oct 9 1992
Externally publishedYes

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Calcium Signaling
Inositol
Heparin
Inositol 1,4,5-Trisphosphate Receptors
Inositol 1,4,5-Trisphosphate
Thapsigargin
Electroporation
Gap Junctions
Epithelial Cells

ASJC Scopus subject areas

  • General

Cite this

Boitano, S. A., Dirksen, E. R., & Sanderson, M. J. (1992). Intercellular propagation of calcium waves mediated by inositol trisphosphate. Science, 258(5080), 292-295.

Intercellular propagation of calcium waves mediated by inositol trisphosphate. / Boitano, Scott A; Dirksen, Ellen R.; Sanderson, Michael J.

In: Science, Vol. 258, No. 5080, 09.10.1992, p. 292-295.

Research output: Contribution to journalArticle

Boitano, SA, Dirksen, ER & Sanderson, MJ 1992, 'Intercellular propagation of calcium waves mediated by inositol trisphosphate', Science, vol. 258, no. 5080, pp. 292-295.
Boitano, Scott A ; Dirksen, Ellen R. ; Sanderson, Michael J. / Intercellular propagation of calcium waves mediated by inositol trisphosphate. In: Science. 1992 ; Vol. 258, No. 5080. pp. 292-295.
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