Interferon-α alters spectrin organization in normal and leukemic human B lymphocytes

Sharon S. Evans, Wan Chao Wang, Carol Gregorio, Tin Han, Elizabeth A. Repasky

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Interferon-α (IFN-α) regulates the growth, differentiation, and recirculation of normal and malignant B lymphocytes. In this report we examine the effects of IFN-α on the distribution of the cytoskeletal protein spectrin in peripheral blood B lymphocytes from normal donors and patients diagnosed with chronic lymphocytic leukemia (CLL) and hairy cell leukemia (HCL). Exposure of normal and leukemic B cells to IFN-α in vitro was shown by immunofluorescence microscopy to cause a dose-dependent increase in the percentage of cells containing discrete focal accumulations of spectrin, ie, a single large aggregate or cap-like structure near the plasma membrane. Although the magnitude of this effect was variable among individual patient samples, in some experiments IFN-α induced a fourfold increase in the percentage of leukemic B cells exhibiting focal accumulations of spectrin. Spectrin reorganization induced by IFN-a was abrogated by the protein synthesis inhibitor cycloheximide. In addition, IFN-α increased the total cellular content of spectrin in B-CLL cells by approximately twofold to fourfold. Finally, a role for protein kinase C in mediating the effects of IFN-α on spectrin's organization is implicated by studies in which calphostin C inhibited the IFN-induced focal accumulation of spectrin. Taken together, these studies suggest that the immunomodulatory activities of IFN-α in normal and malignant B cells involve a change in the organization of the spectrin-based cytoskeleton.

Original languageEnglish (US)
Pages (from-to)759-766
Number of pages8
JournalBlood
Volume81
Issue number3
StatePublished - Feb 1 1993
Externally publishedYes

Fingerprint

Spectrin
Lymphocytes
Interferons
B-Lymphocytes
Cells
B-Cell Chronic Lymphocytic Leukemia
Hairy Cell Leukemia
Protein Synthesis Inhibitors
Cytoskeletal Proteins
Cell membranes
Cycloheximide
Cytoskeleton
Fluorescence Microscopy
Protein Kinase C
Microscopic examination
Blood
Cell Membrane
Tissue Donors

ASJC Scopus subject areas

  • Hematology

Cite this

Evans, S. S., Wang, W. C., Gregorio, C., Han, T., & Repasky, E. A. (1993). Interferon-α alters spectrin organization in normal and leukemic human B lymphocytes. Blood, 81(3), 759-766.

Interferon-α alters spectrin organization in normal and leukemic human B lymphocytes. / Evans, Sharon S.; Wang, Wan Chao; Gregorio, Carol; Han, Tin; Repasky, Elizabeth A.

In: Blood, Vol. 81, No. 3, 01.02.1993, p. 759-766.

Research output: Contribution to journalArticle

Evans, SS, Wang, WC, Gregorio, C, Han, T & Repasky, EA 1993, 'Interferon-α alters spectrin organization in normal and leukemic human B lymphocytes', Blood, vol. 81, no. 3, pp. 759-766.
Evans, Sharon S. ; Wang, Wan Chao ; Gregorio, Carol ; Han, Tin ; Repasky, Elizabeth A. / Interferon-α alters spectrin organization in normal and leukemic human B lymphocytes. In: Blood. 1993 ; Vol. 81, No. 3. pp. 759-766.
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