Intermediate filaments

A review of the basic biology

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

This review presents the current knowledge of the basic biology of intermediate filaments, including their phylogenetic distribution and their distribution within mammalian cells. The current understanding of their structure, recently described using recombinant DNA, peptide sequencing, and immunologic methods, is discussed in terms of a revised chemical classification that not only includes the acidic and neutral basic keratins, vimentin, glial fibrillary protein, desmin, and neurofilament, but also includes the more recently related nuclear lamins. This new structural knowledge allows a more rational approach to the diagnostic use and development of polyclonal and monoclonal anti-intermediate filament antibodies. Many of the problems encountered with fixation, cross-reactivity, and epitope masking can be related to these structural concepts.

Original languageEnglish (US)
Pages (from-to)4-16
Number of pages13
JournalAmerican Journal of Surgical Pathology
Volume12
Issue numberSUPPL. 1
StatePublished - 1988

Fingerprint

Intermediate Filaments
Type II Keratin
Lamins
Neurofilament Proteins
Desmin
Recombinant DNA
Vimentin
DNA Sequence Analysis
Neuroglia
Epitopes
Peptides
Antibodies

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine

Cite this

Intermediate filaments : A review of the basic biology. / Nagle, Raymond B.

In: American Journal of Surgical Pathology, Vol. 12, No. SUPPL. 1, 1988, p. 4-16.

Research output: Contribution to journalArticle

@article{4792d6d965ef46a1aa677630895ba8b2,
title = "Intermediate filaments: A review of the basic biology",
abstract = "This review presents the current knowledge of the basic biology of intermediate filaments, including their phylogenetic distribution and their distribution within mammalian cells. The current understanding of their structure, recently described using recombinant DNA, peptide sequencing, and immunologic methods, is discussed in terms of a revised chemical classification that not only includes the acidic and neutral basic keratins, vimentin, glial fibrillary protein, desmin, and neurofilament, but also includes the more recently related nuclear lamins. This new structural knowledge allows a more rational approach to the diagnostic use and development of polyclonal and monoclonal anti-intermediate filament antibodies. Many of the problems encountered with fixation, cross-reactivity, and epitope masking can be related to these structural concepts.",
author = "Nagle, {Raymond B}",
year = "1988",
language = "English (US)",
volume = "12",
pages = "4--16",
journal = "American Journal of Surgical Pathology",
issn = "0147-5185",
publisher = "Lippincott Williams and Wilkins",
number = "SUPPL. 1",

}

TY - JOUR

T1 - Intermediate filaments

T2 - A review of the basic biology

AU - Nagle, Raymond B

PY - 1988

Y1 - 1988

N2 - This review presents the current knowledge of the basic biology of intermediate filaments, including their phylogenetic distribution and their distribution within mammalian cells. The current understanding of their structure, recently described using recombinant DNA, peptide sequencing, and immunologic methods, is discussed in terms of a revised chemical classification that not only includes the acidic and neutral basic keratins, vimentin, glial fibrillary protein, desmin, and neurofilament, but also includes the more recently related nuclear lamins. This new structural knowledge allows a more rational approach to the diagnostic use and development of polyclonal and monoclonal anti-intermediate filament antibodies. Many of the problems encountered with fixation, cross-reactivity, and epitope masking can be related to these structural concepts.

AB - This review presents the current knowledge of the basic biology of intermediate filaments, including their phylogenetic distribution and their distribution within mammalian cells. The current understanding of their structure, recently described using recombinant DNA, peptide sequencing, and immunologic methods, is discussed in terms of a revised chemical classification that not only includes the acidic and neutral basic keratins, vimentin, glial fibrillary protein, desmin, and neurofilament, but also includes the more recently related nuclear lamins. This new structural knowledge allows a more rational approach to the diagnostic use and development of polyclonal and monoclonal anti-intermediate filament antibodies. Many of the problems encountered with fixation, cross-reactivity, and epitope masking can be related to these structural concepts.

UR - http://www.scopus.com/inward/record.url?scp=0023833503&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023833503&partnerID=8YFLogxK

M3 - Article

VL - 12

SP - 4

EP - 16

JO - American Journal of Surgical Pathology

JF - American Journal of Surgical Pathology

SN - 0147-5185

IS - SUPPL. 1

ER -