Interplay between TCR Affinity and Necessity of Coreceptor Ligation: High-Afinity Peptide-MHC/TCR Interaction Overcomes Lack of CD8 Engagement

Samantha E. Kerry, Jennifer Buslepp, Lorraine A. Cramer, Robert Maile, Lucinda L. Hensley, Alma I. Nielsen, Paula Kavathas, Barbara J. Vilen, Edward J. Collins, Jeffrey A. Frelinger

Research output: Contribution to journalArticle

67 Scopus citations

Abstract

CD8 engagement is believed to be a critical event in the activation of naive T cells. In this communication, we address the effects of peptide-MHC (pMHC)/TCR affinity on the necessity of CD8 engagement in T cell activation of primary naive cells. Using two peptides with different measured avidities for the same pMHC-TCR complex, we compared biochemical affinity of pMHC/TCR and the cell surface binding avidity of pMHC/TCR with and without CD8 engagement. We compared early signaling events and later functional activity of naive T cells in the same manner. Although early signaling events are altered, we find that high-affinity pMHC/TCR interactions can overcome the need for CD8 engagement for proliferation and CTL function. An integrated signal overtime allows T cell activation with a high-affinity ligand in the absence of CD8 engagement.

Original languageEnglish (US)
Pages (from-to)4493-4503
Number of pages11
JournalJournal of Immunology
Volume171
Issue number9
DOIs
StatePublished - Oct 1 2003

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Kerry, S. E., Buslepp, J., Cramer, L. A., Maile, R., Hensley, L. L., Nielsen, A. I., Kavathas, P., Vilen, B. J., Collins, E. J., & Frelinger, J. A. (2003). Interplay between TCR Affinity and Necessity of Coreceptor Ligation: High-Afinity Peptide-MHC/TCR Interaction Overcomes Lack of CD8 Engagement. Journal of Immunology, 171(9), 4493-4503. https://doi.org/10.4049/jimmunol.171.9.4493