Interplay between TCR Affinity and Necessity of Coreceptor Ligation: High-Afinity Peptide-MHC/TCR Interaction Overcomes Lack of CD8 Engagement

Samantha E. Kerry, Jennifer Buslepp, Lorraine A. Cramer, Robert Maile, Lucinda L. Hensley, Alma I. Nielsen, Paula Kavathas, Barbara J. Vilen, Edward J. Collins, Jeffrey A Frelinger

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

CD8 engagement is believed to be a critical event in the activation of naive T cells. In this communication, we address the effects of peptide-MHC (pMHC)/TCR affinity on the necessity of CD8 engagement in T cell activation of primary naive cells. Using two peptides with different measured avidities for the same pMHC-TCR complex, we compared biochemical affinity of pMHC/TCR and the cell surface binding avidity of pMHC/TCR with and without CD8 engagement. We compared early signaling events and later functional activity of naive T cells in the same manner. Although early signaling events are altered, we find that high-affinity pMHC/TCR interactions can overcome the need for CD8 engagement for proliferation and CTL function. An integrated signal overtime allows T cell activation with a high-affinity ligand in the absence of CD8 engagement.

Original languageEnglish (US)
Pages (from-to)4493-4503
Number of pages11
JournalJournal of Immunology
Volume171
Issue number9
StatePublished - Oct 1 2003
Externally publishedYes

Fingerprint

Ligation
Peptides
T-Lymphocytes
Ligands

ASJC Scopus subject areas

  • Immunology

Cite this

Interplay between TCR Affinity and Necessity of Coreceptor Ligation : High-Afinity Peptide-MHC/TCR Interaction Overcomes Lack of CD8 Engagement. / Kerry, Samantha E.; Buslepp, Jennifer; Cramer, Lorraine A.; Maile, Robert; Hensley, Lucinda L.; Nielsen, Alma I.; Kavathas, Paula; Vilen, Barbara J.; Collins, Edward J.; Frelinger, Jeffrey A.

In: Journal of Immunology, Vol. 171, No. 9, 01.10.2003, p. 4493-4503.

Research output: Contribution to journalArticle

Kerry, SE, Buslepp, J, Cramer, LA, Maile, R, Hensley, LL, Nielsen, AI, Kavathas, P, Vilen, BJ, Collins, EJ & Frelinger, JA 2003, 'Interplay between TCR Affinity and Necessity of Coreceptor Ligation: High-Afinity Peptide-MHC/TCR Interaction Overcomes Lack of CD8 Engagement', Journal of Immunology, vol. 171, no. 9, pp. 4493-4503.
Kerry, Samantha E. ; Buslepp, Jennifer ; Cramer, Lorraine A. ; Maile, Robert ; Hensley, Lucinda L. ; Nielsen, Alma I. ; Kavathas, Paula ; Vilen, Barbara J. ; Collins, Edward J. ; Frelinger, Jeffrey A. / Interplay between TCR Affinity and Necessity of Coreceptor Ligation : High-Afinity Peptide-MHC/TCR Interaction Overcomes Lack of CD8 Engagement. In: Journal of Immunology. 2003 ; Vol. 171, No. 9. pp. 4493-4503.
@article{ebf688cb4a164630bbba107d52d87fdf,
title = "Interplay between TCR Affinity and Necessity of Coreceptor Ligation: High-Afinity Peptide-MHC/TCR Interaction Overcomes Lack of CD8 Engagement",
abstract = "CD8 engagement is believed to be a critical event in the activation of naive T cells. In this communication, we address the effects of peptide-MHC (pMHC)/TCR affinity on the necessity of CD8 engagement in T cell activation of primary naive cells. Using two peptides with different measured avidities for the same pMHC-TCR complex, we compared biochemical affinity of pMHC/TCR and the cell surface binding avidity of pMHC/TCR with and without CD8 engagement. We compared early signaling events and later functional activity of naive T cells in the same manner. Although early signaling events are altered, we find that high-affinity pMHC/TCR interactions can overcome the need for CD8 engagement for proliferation and CTL function. An integrated signal overtime allows T cell activation with a high-affinity ligand in the absence of CD8 engagement.",
author = "Kerry, {Samantha E.} and Jennifer Buslepp and Cramer, {Lorraine A.} and Robert Maile and Hensley, {Lucinda L.} and Nielsen, {Alma I.} and Paula Kavathas and Vilen, {Barbara J.} and Collins, {Edward J.} and Frelinger, {Jeffrey A}",
year = "2003",
month = "10",
day = "1",
language = "English (US)",
volume = "171",
pages = "4493--4503",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "9",

}

TY - JOUR

T1 - Interplay between TCR Affinity and Necessity of Coreceptor Ligation

T2 - High-Afinity Peptide-MHC/TCR Interaction Overcomes Lack of CD8 Engagement

AU - Kerry, Samantha E.

AU - Buslepp, Jennifer

AU - Cramer, Lorraine A.

AU - Maile, Robert

AU - Hensley, Lucinda L.

AU - Nielsen, Alma I.

AU - Kavathas, Paula

AU - Vilen, Barbara J.

AU - Collins, Edward J.

AU - Frelinger, Jeffrey A

PY - 2003/10/1

Y1 - 2003/10/1

N2 - CD8 engagement is believed to be a critical event in the activation of naive T cells. In this communication, we address the effects of peptide-MHC (pMHC)/TCR affinity on the necessity of CD8 engagement in T cell activation of primary naive cells. Using two peptides with different measured avidities for the same pMHC-TCR complex, we compared biochemical affinity of pMHC/TCR and the cell surface binding avidity of pMHC/TCR with and without CD8 engagement. We compared early signaling events and later functional activity of naive T cells in the same manner. Although early signaling events are altered, we find that high-affinity pMHC/TCR interactions can overcome the need for CD8 engagement for proliferation and CTL function. An integrated signal overtime allows T cell activation with a high-affinity ligand in the absence of CD8 engagement.

AB - CD8 engagement is believed to be a critical event in the activation of naive T cells. In this communication, we address the effects of peptide-MHC (pMHC)/TCR affinity on the necessity of CD8 engagement in T cell activation of primary naive cells. Using two peptides with different measured avidities for the same pMHC-TCR complex, we compared biochemical affinity of pMHC/TCR and the cell surface binding avidity of pMHC/TCR with and without CD8 engagement. We compared early signaling events and later functional activity of naive T cells in the same manner. Although early signaling events are altered, we find that high-affinity pMHC/TCR interactions can overcome the need for CD8 engagement for proliferation and CTL function. An integrated signal overtime allows T cell activation with a high-affinity ligand in the absence of CD8 engagement.

UR - http://www.scopus.com/inward/record.url?scp=10744227843&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=10744227843&partnerID=8YFLogxK

M3 - Article

C2 - 14568922

AN - SCOPUS:10744227843

VL - 171

SP - 4493

EP - 4503

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 9

ER -