Intracellular cytokine staining for TGF-β

Mohammed L. Garba, Jeffrey A. Frelinger

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

TGF-β is a well-described immunoregulatory molecule that is produced by most cell types. Many studies have been aimed at investigating the role played by TGF-β in different cell types and situations. Most methods of measuring TGF-β have previously relied on enzyme-linked immunosorbent assay (ELISA) or assays of its anti-proliferative effects on various cell lines. Both assays, though useful, cannot be used to effectively identify the cells that are producing TGF-β in a mixture of cells. It is especially important to know the source and dynamics of TGF-β secretion in cell culture studies since most cell types are known to be capable of producing TGF-β. We describe here a technique of qualitative and quantitative measurement of TGF-β production using flow cytometry. Previous work by others has led to the production of polyclonal and monoclonal antibodies to human and other mammalian TGF-β. We have developed an intracellular cytokine staining for human TGF-β using a monoclonal antibody, TB21.

Original languageEnglish (US)
Pages (from-to)193-198
Number of pages6
JournalJournal of Immunological Methods
Volume258
Issue number1-2
DOIs
StatePublished - Dec 1 2001
Externally publishedYes

Keywords

  • Cell line
  • Flow cytometry
  • Intracellular
  • T cells
  • TGF-β

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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