Intracellular in snake renal proximal tubules

Kyu Kim Yung Kyu Kim, W. H. Dantzler

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

Intracellular pH (pH(i)) was studied in isolated proximal renal tubules of garter snakes (Thamnophis spp.) with oil-filled lumens under control conditions [N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid (HEPES)- buffered medium with pH 7.4 at 25°C] and in response to NH4Cl pulse. pH(i) was measured with the pH-sensitive fluorescent dye 2',7'-bis(2-carboxyethyl)- 5,6-carboxyfluorescein (BCECF). Control resting pH(i) (7.1) and acidification in response to NH4Cl pulse (minimum ph(i), 6.6) were essentially the same in snake tubules with oil-filled lumens or perfused lumens and in rabbit S2 proximal tubules with oil filled lumens. Rate of recovery of pH(i) (dpH(i)/dt) from acid to resting level in snake tubules (2.5 x 10-3 pH U/s was about one-third of that in rabbit tubules. Resting pH(i) and dpH(i)/dt from acid to resting level were Na+ dependent in the distal portion but not the proximal portion of snake proximal tubules. However, dpH(i)/dt was not influenced by amiloride or 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid in snake proximal tubules, suggesting that the effect of Na+ on dpH(i)/dt and resting pH(i) may involve membrane potential. This study also indicates that oil-filled lumens do not interfere with measurements of resting pH(i) and do permit evaluation of pH(i) regulation at the basolateral membrane without complications from transport at the luminal membrane.

Original languageEnglish (US)
Pages (from-to)R822-R829
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume269
Issue number4 38-4
StatePublished - Jan 1 1995

Keywords

  • Thamnophis spp.
  • ammonium chloride pulse
  • garter snakes
  • intracellular acidification

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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