Intracellular light-induced release of signaling molecules from gold-coated liposomes

Gabriel V. Orsinger, Joshua D. Williams, Marek Romanowski

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

The combination of laser light and composite nanovesicles enables unique opportunities for precise delivery to, and ondemand release of molecular compounds within, single cells at high spatiotemporal resolution. Here, we demonstrate precise delivery and intracellular release of molecules from gold-coated liposomes via near infrared (NIR) light. The plasmon resonant gold shell provides a light-sensitive trigger for on-demand content release from thermosensitive liposomes. Two demonstrations of intracellular delivery and release from gold-coated liposomes are presented here. The first example uses microinjection to preload gold-coated liposomes into a single cell, followed by exposure to onresonant NIR laser light to trigger release of a fluorescent nuclear dye intracellularly. In the second delivery and release demonstration, gold-coated liposomes encapsulating inositol trisphosphate (IP3), a ubiquitous secondary messenger in cell signaling cascades, passively accumulate within cells via endocytosis. Exposure to on-resonant NIR laser wavelength of light induces rapid release of IP3 from the intracellular liposomes and subsequent activation of Ca2+ signaling at a single cell, monitored by changes in fluorescence intensity of a Ca 2+-sensitive dye.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
PublisherSPIE
Volume8955
ISBN (Print)9780819498687
DOIs
StatePublished - 2014
EventColloidal Nanoparticles for Biomedical Applications IX - San Francisco, CA, United States
Duration: Feb 1 2014Feb 4 2014

Other

OtherColloidal Nanoparticles for Biomedical Applications IX
CountryUnited States
CitySan Francisco, CA
Period2/1/142/4/14

Fingerprint

Liposomes
Gold
gold
delivery
Light
Molecules
cells
molecules
Lasers
Infrared lasers
infrared lasers
actuators
dyes
inositols
Coloring Agents
Demonstrations
Dyes
encapsulating
Cell signaling
Microinjections

Keywords

  • Cell signaling
  • Gold nanoparticles
  • Intracellular delivery
  • Liposomes
  • Plasmon resonance
  • Tumor microenvironment

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Orsinger, G. V., Williams, J. D., & Romanowski, M. (2014). Intracellular light-induced release of signaling molecules from gold-coated liposomes. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8955). [89551G] SPIE. https://doi.org/10.1117/12.2040854

Intracellular light-induced release of signaling molecules from gold-coated liposomes. / Orsinger, Gabriel V.; Williams, Joshua D.; Romanowski, Marek.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8955 SPIE, 2014. 89551G.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Orsinger, GV, Williams, JD & Romanowski, M 2014, Intracellular light-induced release of signaling molecules from gold-coated liposomes. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 8955, 89551G, SPIE, Colloidal Nanoparticles for Biomedical Applications IX, San Francisco, CA, United States, 2/1/14. https://doi.org/10.1117/12.2040854
Orsinger GV, Williams JD, Romanowski M. Intracellular light-induced release of signaling molecules from gold-coated liposomes. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8955. SPIE. 2014. 89551G https://doi.org/10.1117/12.2040854
Orsinger, Gabriel V. ; Williams, Joshua D. ; Romanowski, Marek. / Intracellular light-induced release of signaling molecules from gold-coated liposomes. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8955 SPIE, 2014.
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