Intragenic recombination and diversifying selection contribute to the evolution of downy mildew resistance at the RPP8 locus of arabidopsis

John M. McDowell, Murali Dhandaydham, Terri A. Long, Mark G M Aarts, Stephen A Goff, Eric B. Holub, Jeffery L. Dangl

Research output: Contribution to journalArticle

329 Citations (Scopus)

Abstract

Pathogen resistance (R) genes of the NBS-LRR class (for nucleotide binding site and leucine-rich repeat) are found in many plant species and confer resistance to a diverse spectrum of pathogens. Little is known about the mechanisms that drive NBS-LRR gene evolution in the host-pathogen arms race. We cloned the RPP8 gene (for resistance to Peronospora parasitica) and compared the structure of alleles at this locus in resistant Landsberg erecta (Ler-0) and susceptible Columbia (Col-0) accessions. RPP8-Ler encodes an NBS-LRR protein with a putative N-terminal leucine zipper and is more closely related to previously cloned R genes that confer resistance to bacterial pathogens than it is to other known RPP genes. The RPP8 haplotype in Ler-0 contains the functional RPP8-Ler gene and a nonfunctional homolog, RPH8A. In contrast, the rpp8 locus in Col-0 contains a single chimeric gene, which was likely derived from unequal crossing over between RPP8-Ler and RPH8A ancestors within a Ler-like haplotype. Sequence divergence among RPP8 family members has been accelerated by positive selection on the putative ligand binding region in the LRRs. These observations indicate that NBS-LRR molecular evolution is driven by the same mechanisms that promote rapid sequence diversification among other genes involved in non-self-recognition.

Original languageEnglish (US)
Pages (from-to)1861-1874
Number of pages14
JournalPlant Cell
Volume10
Issue number11
DOIs
StatePublished - Nov 1998
Externally publishedYes

Fingerprint

intragenic recombination
downy mildew
Arabidopsis
Genetic Recombination
Genes
loci
Pathogens
vpr Genes
genes
Haplotypes
pathogens
Peronospora
Leucine Zippers
haplotypes
Molecular Evolution
Peronospora parasitica
Leucine
leucine zipper
crossing over
Nucleotides

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Intragenic recombination and diversifying selection contribute to the evolution of downy mildew resistance at the RPP8 locus of arabidopsis. / McDowell, John M.; Dhandaydham, Murali; Long, Terri A.; Aarts, Mark G M; Goff, Stephen A; Holub, Eric B.; Dangl, Jeffery L.

In: Plant Cell, Vol. 10, No. 11, 11.1998, p. 1861-1874.

Research output: Contribution to journalArticle

McDowell, John M. ; Dhandaydham, Murali ; Long, Terri A. ; Aarts, Mark G M ; Goff, Stephen A ; Holub, Eric B. ; Dangl, Jeffery L. / Intragenic recombination and diversifying selection contribute to the evolution of downy mildew resistance at the RPP8 locus of arabidopsis. In: Plant Cell. 1998 ; Vol. 10, No. 11. pp. 1861-1874.
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