Intrahost mechanisms governing emergence of resistance-breaking variants of Potato virus Y

Rodolfo Acosta-Leal, Zhongguo Xiong

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The emergence of resistance breaking (RB) variants starting from the avirulent Potato virus Y NN strain (PVYNN) was analyzed after imposing different selective host constraints. Tobacco resistance to PVYNN is conferred by va in both NC745 and VAM genotypes, but VAM carries an extra resistance gene, va2. RB-variants emerged only in NC745 and unexpectedly accumulated higher in the original host, tobacco B21, than the parental PVYNN. However, the recovery of RB-variants was interfered by PVYNN in mixed infections. Further analysis indicated that RB-variants also arose in tobacco VAM, but they were limited to subliminal local infections. Their inability to breakout was associated with absence of a mutational adaptation in the viral VPg gene, which implied a loss of fitness in tobacco B21. Altogether, the emergence of RB-variants was conditioned by inherited host constraints, interference by co-infecting avirulent virus genotypes, and fitness tradeoff of virus adaptations.

Original languageEnglish (US)
Pages (from-to)39-47
Number of pages9
JournalVirology
Volume437
Issue number1
DOIs
StatePublished - Mar 1 2013

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Potyvirus
Tobacco
Genotype
Viruses
Viral Genes
Coinfection
Infection
Genes

Keywords

  • Cell-to-cell movement
  • Clonal interference
  • EIF4E
  • Hidden virulence
  • Potyvirus
  • Quasispecies
  • Random drift
  • Recessive resistance
  • Replicative fidelity
  • Selection

ASJC Scopus subject areas

  • Virology

Cite this

Intrahost mechanisms governing emergence of resistance-breaking variants of Potato virus Y. / Acosta-Leal, Rodolfo; Xiong, Zhongguo.

In: Virology, Vol. 437, No. 1, 01.03.2013, p. 39-47.

Research output: Contribution to journalArticle

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