Intraoperative brain tumor resection with indocyanine green using augmented microscopy

Jeffrey R. Watson, Nikolay Martirosyan, Gerald M Lemole, Theodore "Ted" Trouard, Marek Romanowski

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Treatment outcomes for brain cancer have seen dismal improvements over the last two decades as evident in available statistical data. Efforts to address this challenge include development of near-infrared contrast agents for improvements in diagnostic and therapeutic modalities. This creates a need for imaging technologies that can support the intraoperative use of such agents. Here, we report implementation of a recently introduced augmented microscope in combination with indocyanine green (ICG), a near-infrared contrast agent, for surgical image guidance of a glioma resection in a rat model. Luc-C6 cells were implanted in rats in the left-frontal lobe and grown for 22 days. Surgical resection was performed by a neurosurgeon using the augmented microscope with ICG contrast. ICG accumulated in the tumor tissue due to enhanced permeation and retention from the compromised blood-brain barrier. Videos and images were acquired to evaluate image quality and resection margins. The augmented microscope highlighted tumor tissue regions via visualization of ICG fluorescence and was capable of guiding the rat glioma resection.

Original languageEnglish (US)
Article number090501
JournalJournal of Biomedical Optics
Volume23
Issue number9
DOIs
StatePublished - Sep 1 2018

Fingerprint

Indocyanine Green
rats
brain
Rats
Tumors
Brain
Microscopic examination
Microscopes
tumors
microscopes
microscopy
Tissue
Contrast Media
blood-brain barrier
Infrared radiation
Permeation
lobes
Image quality
margins
Visualization

Keywords

  • augmented microscopy
  • brain surgery
  • fluorescence
  • glioma
  • indocyanine green
  • vascular

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Cite this

Intraoperative brain tumor resection with indocyanine green using augmented microscopy. / Watson, Jeffrey R.; Martirosyan, Nikolay; Lemole, Gerald M; Trouard, Theodore "Ted"; Romanowski, Marek.

In: Journal of Biomedical Optics, Vol. 23, No. 9, 090501, 01.09.2018.

Research output: Contribution to journalArticle

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