Intraperitoneal injections as an alternative method for micro-CT contrast enhanced detection of murine liver tumors

Nathan Sweeney, Stephen Marchant, Jesse D. Martinez

Research output: Contribution to journalArticle

Abstract

Micro-computed tomography (micro-CT) coupled with tissue, or vascular, specific contrast agent has emerged as a powerful tool for detecting and monitoring tumor growth in the liver of murine animals. Intravenous injections of contrast agents can be technically challenging and lead to errors that can considerably influence the outcome of a preclinical study, prompting an alternative method. Here we assessed the effectiveness of intraperitoneal injections of polyiodinated triglycerides emulsions (Fenestra LC) in micro-CT imaging of young SCID (8 weeks) and old BALB/c (48 weeks) mice with xenografit or carcinogen-induced liver tumors, respectively, and determined an optimal acquisition time. Utilizing an intraperitoneal injection is a viable alternative administration route for using Fenestra in detection and quantification of murine liver tumor burden.

Original languageEnglish (US)
Pages (from-to)214-217
Number of pages4
JournalBioTechniques
Volume66
Issue number5
DOIs
StatePublished - 2019

Keywords

  • Fenestra LC
  • Intraperitoneal
  • Intravenous
  • Liver cancer
  • Longitudinal
  • Mice
  • MicroCT

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology(all)

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