Intratumoural heterogeneity of 1p deletions and MYCN amplication in neuroblastomas

Jo Vandesompele, Frank Speleman, Nadine Van Roy, Genevive Laureys, Christian Brinkschmidt, Holger Christiansen, Fritz Lampert, Maria Lastowska, Nick Bown, Andy Pearson, James C. Nicholson, Fiona Ross, Valrie Combaret, Olivier Delattre, Bert G. Feuerstein, Dominique Plantaz

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

Background. At least three genetic hallmarks identify aggressive tumour behaviour in neuroblastomas; amplification of the oncogene MYCN; deletion (loss of heterozygosity [LOH]) at the short arm of chromosome 1 (del 1 p36), seen in approximately 28% of the cases; and di-tetraploidy. The MYCN oncogene is amplified in approximately 23% of all neuroblastomas and becomes important for the stratification of therapy in localised and 4s tumours. Up to now, it has been believed that the genetic constellation of neuroblastic tumours is stable and does not alter during tumour evolution or during tumour progression. Procedure. Using fluorescence in situ hybridisation techniques (FISH) to investigate different tumour areas on touch preparations and histological sections, we show that genetic heterogeneity can be detected in neuroblastomas, especially in tumours detected by urinary mass screening. Conclusion. The identification of such cell clones is important, because the MYCN amplification and/or the deletion at 1p36 appear to be responsible for aggressive local growth and development of metastases.

Original languageEnglish (US)
Pages (from-to)1-4
Number of pages4
JournalMedical and Pediatric Oncology
Volume36
Issue number1
DOIs
StatePublished - Jan 27 2001
Externally publishedYes

Keywords

  • Deletion 1p36
  • FISH
  • Genetic evolution
  • Genetics
  • Intratumoural heterogeneity
  • MYCN amplification
  • Neuroblastoma

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Oncology
  • Cancer Research

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    Vandesompele, J., Speleman, F., Van Roy, N., Laureys, G., Brinkschmidt, C., Christiansen, H., Lampert, F., Lastowska, M., Bown, N., Pearson, A., Nicholson, J. C., Ross, F., Combaret, V., Delattre, O., Feuerstein, B. G., & Plantaz, D. (2001). Intratumoural heterogeneity of 1p deletions and MYCN amplication in neuroblastomas. Medical and Pediatric Oncology, 36(1), 1-4. https://doi.org/10.1002/1096-911X(20010101)36:1<1::AID-MPO1002>3.0.CO;2-L