Intravitreal clearance of tissue plasminogen activator in the rabbit

G. J. Jaffe, G. D J Green, Brian S Mckay, A. Hartz, G. A. Williams

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The clearance of tissue plasminogen activator (t-PA) injected into the midvitreous cavity was studied in the phakic, vitrectomized rabbit eye with and without intravitreal fibrin clots. The quantity and activity of t-PA in the vitreous, serum, and aqueous were determined at ten minutes and at 3, 6, 15, 24, and 48 hours after initial injection by an enzyme-linked immunosorbent assay (ELISA) and a spectrophotometric solid-phase fibrin assay (SOFIA). In eyes without an intravitreal fibrin clot, the estimated half-life for t-PA was 4.3 hours by SOFIA and 5.8 hours by ELISA. In eyes containing a vitreal fibrin clot, the half-life increased to 9.8 hours by SOFIA and 11.9 hours by ELISA. Both of these half-lives were significantly greater than the half-life for eyes without fibrin. Regardless of the presence of fibrin, intravitreal t-PA activity was significantly less than t-PA quantity, suggesting the presence of a t-PA inhibitor. A peak in aqueous t-PA occurred before six hours, indicating that t-PA was cleared in part through the anterior chamber. There was no measurable serum t-PA at any of the sampling times.

Original languageEnglish (US)
Pages (from-to)969-972
Number of pages4
JournalArchives of Ophthalmology
Volume106
Issue number7
StatePublished - 1988
Externally publishedYes

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Tissue Plasminogen Activator
Fibrin
Rabbits
Half-Life
Enzyme-Linked Immunosorbent Assay
Plasminogen Inactivators
Anterior Chamber
Serum
Injections

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Jaffe, G. J., Green, G. D. J., Mckay, B. S., Hartz, A., & Williams, G. A. (1988). Intravitreal clearance of tissue plasminogen activator in the rabbit. Archives of Ophthalmology, 106(7), 969-972.

Intravitreal clearance of tissue plasminogen activator in the rabbit. / Jaffe, G. J.; Green, G. D J; Mckay, Brian S; Hartz, A.; Williams, G. A.

In: Archives of Ophthalmology, Vol. 106, No. 7, 1988, p. 969-972.

Research output: Contribution to journalArticle

Jaffe, GJ, Green, GDJ, Mckay, BS, Hartz, A & Williams, GA 1988, 'Intravitreal clearance of tissue plasminogen activator in the rabbit', Archives of Ophthalmology, vol. 106, no. 7, pp. 969-972.
Jaffe, G. J. ; Green, G. D J ; Mckay, Brian S ; Hartz, A. ; Williams, G. A. / Intravitreal clearance of tissue plasminogen activator in the rabbit. In: Archives of Ophthalmology. 1988 ; Vol. 106, No. 7. pp. 969-972.
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