Intrinsic and synaptic homeostatic plasticity in motoneurons from mice with glycine receptor mutations

M. A. Tadros, K. E. Farrell, P. R. Schofield, A. M. Brichta, B. A. Graham, Andrew J Fuglevand, R. J. Callister

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Inhibitory synaptic inputs to hypoglossal motoneurons (HMs) are important for modulating excitability in brainstem circuits. Here we ask whether reduced inhibition, as occurs in three murine mutants with distinct naturally occurring mutations in the glycine receptor (GlyR), leads to intrinsic and/or synaptic homeostatic plasticity. Whole cell recordings were obtained from HMs in transverse brainstem slices from wild-type (wt), spasmodic (spd), spastic (spa), and oscillator (ot) mice (C57Bl/6, approximately postnatal day 21). Passive and action potential (AP) properties in spd and ot HMs were similar to wt. In contrast, spa HMs had lower input resistances, more depolarized resting membrane potentials, higher rheobase currents, smaller AP amplitudes, and slower afterhyperpolarization current decay times. The excitability of HMs, assessed by "gain" in injected current/firing-frequency plots, was similar in all strains whereas the incidence of rebound spiking was increased in spd. The difference between recruitment and derecruitment current (i.e., ΔI) for AP discharge during ramp current injection was more negative in spa and ot. GABAA miniature inhibitory postsynaptic current (mIPSC) amplitude was increased in spa and ot but not spd, suggesting diminished glycinergic drive leads to compensatory adjustments in the other major fast inhibitory synaptic transmitter system in these mutants. Overall, our data suggest long-term reduction in glycinergic drive to HMs results in changes in intrinsic and synaptic properties that are consistent with homeostatic plasticity in spa and ot but not in spd. We propose such plasticity is an attempt to stabilize HM output, which succeeds in spa but fails in ot.

Original languageEnglish (US)
Pages (from-to)1487-1498
Number of pages12
JournalJournal of Neurophysiology
Volume111
Issue number7
DOIs
StatePublished - Apr 1 2014

Fingerprint

Glycine Receptors
Neuronal Plasticity
Motor Neurons
Muscle Spasticity
Mutation
Action Potentials
Brain Stem
Inhibitory Postsynaptic Potentials
Architectural Accessibility
Patch-Clamp Techniques
Membrane Potentials
Injections
Incidence

Keywords

  • Action potential
  • Excitability
  • GABA

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)
  • Medicine(all)

Cite this

Tadros, M. A., Farrell, K. E., Schofield, P. R., Brichta, A. M., Graham, B. A., Fuglevand, A. J., & Callister, R. J. (2014). Intrinsic and synaptic homeostatic plasticity in motoneurons from mice with glycine receptor mutations. Journal of Neurophysiology, 111(7), 1487-1498. https://doi.org/10.1152/jn.00728.2013

Intrinsic and synaptic homeostatic plasticity in motoneurons from mice with glycine receptor mutations. / Tadros, M. A.; Farrell, K. E.; Schofield, P. R.; Brichta, A. M.; Graham, B. A.; Fuglevand, Andrew J; Callister, R. J.

In: Journal of Neurophysiology, Vol. 111, No. 7, 01.04.2014, p. 1487-1498.

Research output: Contribution to journalArticle

Tadros, MA, Farrell, KE, Schofield, PR, Brichta, AM, Graham, BA, Fuglevand, AJ & Callister, RJ 2014, 'Intrinsic and synaptic homeostatic plasticity in motoneurons from mice with glycine receptor mutations', Journal of Neurophysiology, vol. 111, no. 7, pp. 1487-1498. https://doi.org/10.1152/jn.00728.2013
Tadros, M. A. ; Farrell, K. E. ; Schofield, P. R. ; Brichta, A. M. ; Graham, B. A. ; Fuglevand, Andrew J ; Callister, R. J. / Intrinsic and synaptic homeostatic plasticity in motoneurons from mice with glycine receptor mutations. In: Journal of Neurophysiology. 2014 ; Vol. 111, No. 7. pp. 1487-1498.
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