Invasion of previously unreported dicot plant hosts by chickpea chlorotic dwarf virus in Pakistan

U. Hameed, M. Zia-Ur-Rehman, S. A. Ali, M. S. Haider, Judith K Brown

Research output: Contribution to journalArticle

Abstract

Members of the genus, Mastrevirus (family, Gemniviridae) transmitted by leafhopper vectors infect monocotyledonous or dicotyledonous plants, and infection of agricultural crops results in reduced yield and quality. During 2012, a study was undertaken in the Punjab and Sindh Provinces in Pakistan to determine the identity of suspect geminiviruses associated with symptomatic cotton and vegetable plants exhibiting foliar enations, leaf curling, mosaic, and stunting reminiscent of geminivirus infection. To determine suspect geminiviral identity, fifteen apparently full-length mastrevirus genome (~ 2600 base pairs) were amplified by rolling-circle amplification, digested, cloned into the plasmid vector, pGEM-3Zf+, and sequenced from cucumber, Gossypium arboreum L., Gossypium hirsutum L., okra and tomato. The mastrevirus full-length genome sequences obtained shared their highest pairwise nucleotide sequence identity, at 97.3–98.6%, with previously reported C and L strains of Chickpea chlorotic dwarf virus (CpCDV) from chickpea and cotton in Pakistan, respectively. However, CpCDV has not been previously identified from cucumber, G. arboreum, okra, or tomato. The association of CpCDV with four previously unreported plant hosts suggests that CpCDV strains C and strain L have a broader than expected host range, and therefore may be found to negatively affect vegetable crops, particularly, when grown in proximity to cotton.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalVirusDisease
DOIs
StateAccepted/In press - May 25 2018

Fingerprint

Geminiviridae
Cicer
Pakistan
Gossypium
Viruses
Abelmoschus
Cucumis sativus
Lycopersicon esculentum
Vegetables
Agricultural Crops
Genome
Growth Disorders
Hemiptera
Host Specificity
Infection
Base Pairing
Plasmids

Keywords

  • Chickpea chlorotic dwarf virus
  • Gossypium arboreum
  • Mastrevirus

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Invasion of previously unreported dicot plant hosts by chickpea chlorotic dwarf virus in Pakistan. / Hameed, U.; Zia-Ur-Rehman, M.; Ali, S. A.; Haider, M. S.; Brown, Judith K.

In: VirusDisease, 25.05.2018, p. 1-6.

Research output: Contribution to journalArticle

Hameed, U. ; Zia-Ur-Rehman, M. ; Ali, S. A. ; Haider, M. S. ; Brown, Judith K. / Invasion of previously unreported dicot plant hosts by chickpea chlorotic dwarf virus in Pakistan. In: VirusDisease. 2018 ; pp. 1-6.
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